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  • 产品名称：MetalloproteinaseInhibitor2(TIMP2)(AA27-220)(Active)protein(Histag)

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  • 产品厂商：ACROBiosystems
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简单介绍：
MetalloproteinaseInhibitor2(TIMP2)(AA27-220)(Active)protein(Histag)
详情介绍：
Characteristics This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 22.6 kDa. The protein migrates as 22 kDa under reducing (R) condition (SDS-PAGE).
Purity >98 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
Background TIMP metallopeptidase inhibitor 2 is also known as TIMP2, which belongs to the protease inhibitor I35 (TIMP) family. This family protein are natural inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix. The TIMP family encompasses four members ( TIMP1, TIMP2, TIMP3, TIMP4 ), and they inhibit most MMPs by forming non-covalent binary complex. In addition to an inhibitory role against metalloproteinases, TIMP2 has a unique role among TIMP family members in its ability to directly suppress the proliferation of endothelial cells. As a result, TIMP-2 may be critical to the maintenance of tissue homeostasis by suppressing the proliferation of quiescent tissues in response to angiogenic factors, and by inhibiting protease activity in tissues undergoing remodelling of the extracellular matrix.
Molecular Weight 22.6 kDa
NCBI Accession NP_003246
UniProt P16035
Research Area Extracellular Matrix, Cancer, Inflammation
Pathways cAMP Metabolic Process
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer 50 mM Tris, 150 mM NaCl, 0.05 % Brij-35, pH 7.5
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C-8 °C), After reconstitution under sterile conditions for 1 month (4 °C-8 °C) or 3 months (-20 °C to -70 °C).
Supplier Images
SDS-PAGE (SDS) image for Metalloproteinase Inhibitor 2 (TIMP2) (AA 27-220) (Active) protein (His tag) (ABIN2181824) Human TIMP-2, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained o...
Background publications Chattopadhyay, Mitra, Frei, Chatterjee: "Human cervical tumor cell (SiHa) surface alphavbeta3 integrin receptor has associated matrix metalloproteinase (MMP-2) activity." in: Journal of cancer research and clinical oncology, Vol. 127, Issue 11, pp. 653-8, 2001 (PubMed).

Stetler-Stevenson, Krutzsch, Liotta: "Tissue inhibitor of metalloproteinase (TIMP-2). A new member of the metalloproteinase inhibitor family." in: The Journal of biological chemistry, Vol. 264, Issue 29, pp. 17374-8, 1989 (PubMed).

Goldberg, Marmer, Grant, Eisen, Wilhelm, He: "Human 72-kilodalton type IV collagenase forms a complex with a tissue inhibitor of metalloproteases designated TIMP-2." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 86, Issue 21, pp. 8207-11, 1989 (PubMed).