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  • 产品名称：CD27Molecule(CD27)(AA21-192)(Active)protein(FcTag)

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  • 产品厂商：ACROBiosystems
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简单介绍：
CD27Molecule(CD27)(AA21-192)(Active)protein(FcTag)
详情介绍：
Characteristics This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 45.9 kDa. The protein migrates as 45-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Purity >95 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
ProductDetails: Biological Activity Comment Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized recombinant human CD27 protein at 2 μg/mL (100μL/well) can bind biotinylated human CD70 with a linear range of 0.25 - 16 ng/mL.
Background CD Antigen CD27 is also known as Tumor necrosis factor receptor superfamily member 7 (TNFRSF7), which belongs to TNF-receptor superfamily. CD27 / TNFRSF7 is found in most T-lymphocytes. CD27 / TNFRSF7 is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. This receptor transduces signals that lead to the activation of NF-κB and MAPK8/JNK. CD27 has been shown to interact with SIVA1, TRAF2 and TRAF3.
Molecular Weight 45.9 kDa
NCBI Accession NP_001233
UniProt P26842
Research Area Immunology, Adaptive Immunity, Hematopoietic Progenitors, Cytokines, Innate Immunity, CD Antigens, Surface Receptors of Immune Cells
Pathways Production of Molecular Mediator of Immune Response
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer 50 mM Tris, 100 mM Glycine, pH 7.5
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).
Supplier Images
SDS-PAGE (SDS) image for CD27 Molecule (CD27) (AA 21-192) (Active) protein (Fc Tag) (ABIN2180735) CD27 Molecule (CD27) (AA 21-192) (Active) protein (Fc Tag)
Background publications Zhang, Henzel: "Signal peptide prediction based on analysis of experimentally verified cleavage sites." in: Protein science : a publication of the Protein Society, Vol. 13, Issue 10, pp. 2819-24, 2004 (PubMed).

Gravestein, Amsen, Boes, Calvo, Kruisbeek, Borst: "The TNF receptor family member CD27 signals to Jun N-terminal kinase via Traf-2." in: European journal of immunology, Vol. 28, Issue 7, pp. 2208-16, 1998 (PubMed).

Sattler, Salgia, Shrikhande, Verma, Pisick, Prasad, Griffin: "Steel factor induces tyrosine phosphorylation of CRKL and binding of CRKL to a complex containing c-kit, phosphatidylinositol 3-kinase, and p120(CBL)." in: The Journal of biological chemistry, Vol. 272, Issue 15, pp. 10248-53, 1997 (PubMed).

Prasad, Ao, Yoon, Wu, Rizk, Jacquot, Schlossman: "CD27, a member of the tumor necrosis factor receptor family, induces apoptosis and binds to Siva, a proapoptotic protein." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, Issue 12, pp. 6346-51, 1997 (PubMed).