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  • 产品名称：CarboxypeptidaseE(CPE)(AA26-453)(Active)protein(Histag)

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  • 产品厂商：ACROBiosystems
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简单介绍：
CarboxypeptidaseE(CPE)(AA26-453)(Active)protein(Histag)
详情介绍：
Characteristics This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 49 kDa. The protein migrates as 53 kDa under reducing (R) condition (SDS-PAGE).
Purity >95 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
ProductDetails: Biological Activity Comment Biological Activity: Measured by its ability to cleave a peptide substrate, benzoylAROH. The product, Arg, is reacted with orthophthaldialdehyde (OPA) to form a fluorescent molecule. The specific activity is > 12000 pmoles/min/μg.
Background Carboxypeptidase E (CPE) is also known as Carboxypeptidase H or enkephalin convertase. Carboxypeptidase E cleaves C-terminal amino acid residues and is involved in neuropeptide processing. It is a peripheral membrane protein. CPE specifically binds regulated secretory pathway proteins, including prohormones, but not constitutively secreted proteins. Carboxypeptidase E appears to have several functions. The active form of carboxypeptidase E was shown to be in secretory vesicles, where it acts as an exopeptidase to activate neuropeptides, It does that by cleaving off basic C-terminal amino acids, producing the active form of the peptide. Products of carboxypeptidase E include insulin, enkephalin, and most other neuroendocrine peptides.It has also been proposed that membrane-associated carboxypeptidase E acts as a sorting signal for regulated secretory proteins in the trans-Golgi network of the pituitary and in secretory granules, regulated secretory proteins are mostly hormones and neuropeptides. Mice with mutant carboxypeptidase E Cpe, display endocrine disorders like obesity and infertility.
Molecular Weight 49 kDa
NCBI Accession NP_001864
UniProt P16870
Research Area Enzymes, Metabolism
Pathways Peptide Hormone Metabolism, Synaptic Membrane
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer PBS, pH 7.4
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).
Supplier Images
SDS-PAGE (SDS) image for Carboxypeptidase E (CPE) (AA 26-453) (Active) protein (His tag) (ABIN2180678) Human Carboxypeptidase E, His Tag on SDS-PAGE under reducing (R) condition. The gel w...
Background publications Jeffrey, Alejandro, Luciani, Kalynyak, Hu, Li, Lin, Townsend, Polonsky, Johnson: "Carboxypeptidase E mediates palmitate-induced beta-cell ER stress and apoptosis." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 24, pp. 8452-7, 2008 (PubMed).

Tai, Foster, Rosales, Feng, Hasilo, Martinez, Ramadan, Snir, Melling, Dhanvantari, Rutt, White: "Imaging islets labeled with magnetic nanoparticles at 1.5 Tesla." in: Diabetes, Vol. 55, Issue 11, pp. 2931-8, 2006 (PubMed).

Dhanvantari, Loh: "Lipid raft association of carboxypeptidase E is necessary for its function as a regulated secretory pathway sorting receptor." in: The Journal of biological chemistry, Vol. 275, Issue 38, pp. 29887-93, 2000 (PubMed).

Manser, Fernandez, Loo, Goh, Monfries, Hall, Lim: "Human carboxypeptidase E. Isolation and characterization of the cDNA, sequence conservation, expression and processing in vitro." in: The Biochemical journal, Vol. 267, Issue 2, pp. 517-25, 1990 (PubMed).