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  • 产品名称：CD80(CD80)(AA35-242)(Active)protein(FcTag)

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  • 产品厂商：ACROBiosystems
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简单介绍：
CD80(CD80)(AA35-242)(Active)protein(FcTag)
详情介绍：
Characteristics This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 50 kDa. The protein migrates as 70-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Purity >95 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
Grade HPLC verified
ProductDetails: Biological Activity Comment Biological Activity: Measured by its ability to induce IL-2 secretion by Jurkat human acute T cell leukemia cells.The ED50for this effect is typically 0.025 - 0.15 μg/mL in the presence of PHA. Measured by its binding ability in a functional ELISA. Immobilized rhB7-1/CD80 Protein Fc Chimera (Catalog # B71-H5259) at 2 μg/mL (100 μL/well) can bind Biotinylated rh CTLA4, His.Avi Tag (Catalog # CT4-H82E4) with a linear range of 50 - 500 ng/mL.

SDS-PAGE: due to glycosylation.
Background B7-1 and B7-2, together with their receptors CD28 and CTLA-4, constitute one of the dominant co-stimulatory pathways that regulate T- and B-cell responses. Although both CTLA-4 and CD28 can bind to the same ligands, CTLA-4 binds to B7-1 and B7-2 with a 20 - 100 fold higher affinity than CD28 and is involved in the down-regulation of the immune response. B-lymphocyte activation antigen B7-1 (referred to as B7) also known as cluster of Differentiation 80 (CD80), is a member of cell surface immunoglobulin superfamily and is expressed on activated B cells, activated T cells, macrophages and dendritic cells. It is the ligand for two different proteins on the T cell surface: CD28 (for autoregulation and intercellular association) and CTLA-4 (for attenuation of regulation and cellular disassociation). CD80 works in tandem with CD86 to prime T cells. CD80 plays a role in induction of innate immune responses by activating NF-κB-signaling pathway in macrophages. CD80 is thus regarded as promising therapeutic targets for autoimmune diseases and various carcinomas.
Molecular Weight 50 kDa
NCBI Accession NP_005182
UniProt P33681
Pathways TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Immune Effector Process
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer 50 mM Tris, 100 mM Glycine, pH 7.5
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).
Supplier Images
SDS-PAGE (SDS) image for CD80 (CD80) (AA 35-242) (Active) protein (Fc Tag) (ABIN2180845) Human B7-1, Fc Tag (HPLC-verified) on SDS-PAGE under reducing (R) condition. The gel ...
Binding Studies (Bind) image for CD80 (CD80) (AA 35-242) (Active) protein (Fc Tag) (ABIN2180845) Immobilized Human B7-1, Fc Tag (HPLC-verified) with a linear range of 1-20 ng/mL.
Binding Studies (Bind) image for CD80 (CD80) (AA 35-242) (Active) protein (Fc Tag) (ABIN2180845) Immobilized Human B7-1, Fc Tag (HPLC-verified) with a linear range of 50 - 500 ng/mL.
Background publications Khan, Ghousunnissa, Jegadeeswaran, Thiagarajan, Hasnain, Mukhopadhyay: "Anti-B7-1/B7-2 antibody elicits innate-effector responses in macrophages through NF-kappaB-dependent pathway." in: International immunology, Vol. 19, Issue 4, pp. 477-86, 2007 (PubMed).

Bhatia, Edidin, Almo, Nathenson: "Different cell surface oligomeric states of B7-1 and B7-2: implications for signaling." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 102, Issue 43, pp. 15569-74, 2005 (PubMed).