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  • 产品名称：TumorNecrosisFactorReceptorSuperfamily,Member8(TNFRSF8)(AA19-379)(Active)protei

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  • 产品厂商：ACROBiosystems
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简单介绍：
TumorNecrosisFactorReceptorSuperfamily,Member8(TNFRSF8)(AA19-379)(Active)protein(Histag)
详情介绍：
Characteristics This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 39.3 kDa. The protein migrates as 75-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Purity >95 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
ProductDetails: Biological Activity Comment Biological Activity: Measured by its ability to block CD30 Ligand-induced IL-6 secretion by HDLM human Hodgkin's lymphoma cells. The ED50 for this effect is typically 0.5 - 2.5 μg/mL in the presence of 50 ng/mL of Recombinant Human CD30 Ligand.
Background Human CD30 is also known as TNFRSF8, is a cell membrane protein of the tumor necrosis factor receptor family and tumor marker. TNFRSF-8 is expressed by activated, but not by resting, T and B cells. Also, CD30 is expressed on classical Hodgkin Lymphoma cells together with CD15. CD30 is the receptor for TNFSF8/CD30L. CD30 can interact with TRAF2 and TRAF5, and mediate the signal transduction that leads to the activation of NF-kappa-B. TNFRSF8 may play a role in the regulation of cellular growth and transformation of activated lymphoblasts. TNFRSF8 is a positive regulator of apoptosis, and also has been shown to limit the proliferative potential of autoreactive CD8 effector T cells and protect the body against autoimmunity.
Molecular Weight 39.3 kDa
NCBI Accession NP_001234
UniProt P28908
Research Area Cancer, Innate Immunity, Cytokines, CD Antigens, Surface Receptors of Immune Cells
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer PBS, pH 7.4
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C-8 °C), After reconstitution under sterile conditions for 1 month (4 °C-8 °C) or 3 months (-20 °C to -70 °C).
Supplier Images
SDS-PAGE (SDS) image for Tumor Necrosis Factor Receptor Superfamily, Member 8 (TNFRSF8) (AA 19-379) (Active) protein (His tag) (ABIN2180740) Tumor Necrosis Factor Receptor Superfamily, Member 8 (TNFRSF8) (AA 19-379) (Active) protein (His tag)
Background publications Ansieau, Scheffrahn, Mosialos, Brand, Duyster, Kaye, Harada, Dougall, Hübinger, Kieff, Herrmann, Leutz, Gruss: "Tumor necrosis factor receptor-associated factor (TRAF)-1, TRAF-2, and TRAF-3 interact in vivo with the CD30 cytoplasmic domain; TRAF-2 mediates CD30-induced nuclear factor kappa B activation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 93, Issue 24, pp. 14053-8, 1997 (PubMed).

Lee, Park, Choi: "T cell receptor-dependent cell death of T cell hybridomas mediated by the CD30 cytoplasmic domain in association with tumor necrosis factor receptor-associated factors." in: The Journal of experimental medicine, Vol. 183, Issue 2, pp. 669-74, 1996 (PubMed).