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  • 产品名称：Glypican3(GPC3)(AA25-559)(Active)protein(Histag)

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  • 产品厂商：ACROBiosystems
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简单介绍：
Glypican3(GPC3)(AA25-559)(Active)protein(Histag)
详情介绍：
Characteristics This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 61.7 kDa. The protein migrates as 40 kDa and 60-130 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation and associated via disulfide bonds.
Purity >90 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 0.1 EU per μg by the LAL method.
ProductDetails: Biological Activity Comment Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized rh GPC3 / Glypican-3 at 5 μg/mL ( 100 μL/well ) can bind human bFGF / FGF2 (Catalog # BFF-H4117) with a linear ranger of 0.16 - 3.2 ng/mL.

SDS-PAGE: due to glycosylation and associated via disulfide bonds.
Background Glypican-3 (GPC3) is also known as Intestinal protein OCI-5, GTR2-2, MXR7, which belongs to the glypican family. Glypican 3 / GPC-3 is highly expressed in lung, liver and kidney. Glypican-3 inhibits the dipeptidyl peptidase activity of DPP4. Glypican-3 may be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs, and also may play a role in the modulation of IGF2 interactions with its receptor and thereby modulate its function.
Molecular Weight 61.7 kDa
NCBI Accession NP_004475
UniProt P51654
Research Area Lineage Markers
Pathways Glycosaminoglycan Metabolic Process
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer PBS, pH 7.4
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).
Supplier Images
SDS-PAGE (SDS) image for Glypican 3 (GPC3) (AA 25-559) (Active) protein (His tag) (ABIN2181182) Human Glypican 3, His Tag on SDS-PAGE under reducing (R) condition. The gel was stain...
Background publications Davoodi, Kelly, Gendron, MacKenzie: "The Simpson-Golabi-Behmel syndrome causative glypican-3, binds to and inhibits the dipeptidyl peptidase activity of CD26." in: Proteomics, Vol. 7, Issue 13, pp. 2300-10, 2007 (PubMed).

Veugelers, Cat, Muyldermans, Reekmans, Delande, Frints, Legius, Fryns, Schrander-Stumpel, Weidle, Magdalena, David: "Mutational analysis of the GPC3/GPC4 glypican gene cluster on Xq26 in patients with Simpson-Golabi-Behmel syndrome: identification of loss-of-function mutations in the GPC3 gene." in: Human molecular genetics, Vol. 9, Issue 9, pp. 1321-8, 2000 (PubMed).