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  • 产品名称：MHCClassIPolypeptide-RelatedSequenceA(MICA)(AA24-308)(Active)protein(Histag)

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  • 产品厂商：ACROBiosystems
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简单介绍：
MHCClassIPolypeptide-RelatedSequenceA(MICA)(AA24-308)(Active)protein(Histag)
详情介绍：
Characteristics This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 33.6 kDa. The protein migrates as 45-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Purity >95 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
Background MHC class I polypeptide-related sequence A (MICA) belongs to the MHC class I family and MIC subfamily. MICA contains one Ig-like C1-type (immunoglobulin-like) domain. Unlike classical MHC class I molecules, MICA does not form a heterodimer with beta-2-microglobulin. MICA acts as a stress-induced self-antigen that is recognized by gamma delta T-cells. MICA is ligand for the KLRK1/NKG2D receptor. MICA bind to KLRK1 leads to cell lysis.
Molecular Weight 33.6 kDa
NCBI Accession NP_000238
UniProt Q29983
Research Area Immunology, Innate Immunity, Adaptive Immunity
Pathways Activation of Innate immune Response, Transition Metal Ion Homeostasis
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer PBS, pH 7.4
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).
Supplier Images
SDS-PAGE (SDS) image for MHC Class I Polypeptide-Related Sequence A (MICA) (AA 24-308) (Active) protein (His tag) (ABIN2181502) Human MICA, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained ove...
Background publications Steinle, Li, Morris, Groh, Lanier, Strong, Spies: "Interactions of human NKG2D with its ligands MICA, MICB, and homologs of the mouse RAE-1 protein family." in: Immunogenetics, Vol. 53, Issue 4, pp. 279-87, 2001 (PubMed).

Bauer, Groh, Wu, Steinle, Phillips, Lanier, Spies: "Activation of NK cells and T cells by NKG2D, a receptor for stress-inducible MICA." in: Science (New York, N.Y.), Vol. 285, Issue 5428, pp. 727-9, 1999 (PubMed).

Groh, Steinle, Bauer, Spies: "Recognition of stress-induced MHC molecules by intestinal epithelial gammadelta T cells." in: Science (New York, N.Y.), Vol. 279, Issue 5357, pp. 1737-40, 1998 (PubMed).