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  • 产品名称：LeukocyteImmunoglobulin-LikeReceptor,SubfamilyB(WithTMandITIMDomains),Member1(L

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  • 产品厂商：ACROBiosystems
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简单介绍：
LeukocyteImmunoglobulin-LikeReceptor,SubfamilyB(WithTMandITIMDomains),Member1(LILRB1)(AA24-458)(Active)protein(FcTag)
详情介绍：
Characteristics This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 73.8 kDa. The protein migrates as 90-110 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Purity >92 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
ProductDetails: Biological Activity Comment Biological Activity: Measured by its ability to support the adhesion of HSB2 human peripheral blood acute lymphoblastic leukemia cells. Immobilized rhCD85J / LILRB1 Fc Chimera at 10 μg/mL, 100 μL/well can support 60-80% HSB2 cell adhesion, when 100000 cells were added in each well of a 96-well plate.
Background CD85 antigen-like family member J (CD85J) is also known as Leukocyte immunoglobulin-like receptor subfamily B member 1 (LILRB1 or LIR-1), Immunoglobulin-like transcript 2 (ILT-2), Monocyte/macrophage immunoglobulin-like receptor 7 (MIR7), which belongs to leukocyte immunoglobulin-like receptor (LIR) family. CD85J / LILRB1 Contains 4 Ig-like C2-type (immunoglobulin-like) domains. CD85J / LIR-1 is expressed predominantly on B-cells and monocytes. CD85J is receptor for class I MHC antigens and recognizes a broad spectrum of HLA-A, HLA-B, HLA-C and HLA-G alleles and is also receptor for H301/UL18, a human cytomegalovirus class I MHC homolog. Ligand binding results in inhibitory signals and down-regulation of the immune response. CD85J / LILRB1 interaction with HLA-B or HLA-E leads to inhibition of the signal triggered by FCER1A and inhibits serotonin release. CD85J / LILRB1 inhibits FCGR1A-mediated phosphorylation of cellular proteins and mobilization of intracellular calcium ions.
Molecular Weight 73.8 kDa
NCBI Accession NP_006660
UniProt Q8NHL6
Research Area Immunology, Adaptive Immunity
Pathways Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer 50 mM Tris, 100 mM Glycine, pH 7.5
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C-8 °C), After reconstitution under sterile conditions for 1 month (4 °C-8 °C) or 3 months (-20 °C to -70 °C).
Supplier Images
SDS-PAGE (SDS) image for Leukocyte Immunoglobulin-Like Receptor, Subfamily B (With TM and ITIM Domains), Member 1 (LILRB1) (AA 24-458) (Active) protein (Fc Tag) (ABIN2181470) Human LILRB1, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained ov...
Background publications Bellón, Kitzig, Sayós, López-Botet: "Mutational analysis of immunoreceptor tyrosine-based inhibition motifs of the Ig-like transcript 2 (CD85j) leukocyte receptor." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 168, Issue 7, pp. 3351-9, 2002 (PubMed).

Chapman, Heikeman, Bjorkman: "The inhibitory receptor LIR-1 uses a common binding interaction to recognize class I MHC molecules and the viral homolog UL18." in: Immunity, Vol. 11, Issue 5, pp. 603-13, 2000 (PubMed).

Fanger, Cosman, Peterson, Braddy, Maliszewski, Borges: "The MHC class I binding proteins LIR-1 and LIR-2 inhibit Fc receptor-mediated signaling in monocytes." in: European journal of immunology, Vol. 28, Issue 11, pp. 3423-34, 1998 (PubMed).

Cosman, Fanger, Borges, Kubin, Chin, Peterson, Hsu: "A novel immunoglobulin superfamily receptor for cellular and viral MHC class I molecules." in: Immunity, Vol. 7, Issue 2, pp. 273-82, 1997 (PubMed).