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  • 产品名称：alpha-2-HS-Glycoprotein(AHSG)(AA19-367)(Active)protein(Histag)

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  • 产品厂商：ACROBiosystems
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简单介绍：
alpha-2-HS-Glycoprotein(AHSG)(AA19-367)(Active)protein(Histag)
详情介绍：
Characteristics This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 38.2 kDa. The protein migrates as 55-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Purity >98 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
ProductDetails: Biological Activity Comment Biological Activity: Measured by its ability to inhibit calcium phosphate precipitation in 100μL reaction mixture containing 4.8 mM Calcium chloride, 1.6 mM Sodium phosphate, with the rhFetuin A concentration curve of 250, 125, 62.5, 31.3, 15.6, 7.8, 3.9, 1.95, 0.97 and 0.49 μg/mL. The IC50 value is <45 μg="" ml.="" <="" td="">
Background Alpha-2-HS-glycoprotein (AHSG) is also known as Alpha-2-Z-globulin, Ba-alpha-2-glycoprotein, Fetuin-A (FETUA), is a glycoprotein that is composed of two subunits, the A and B chains, belongs to the cystatin family of proteases inhibitors and the fetuin class of plasma binding proteins. AHSG / Fetuin A is highly expressed in embryonic cells and ***** hepatocytes, and is expressed to a lesser extent in monocytes/macrophages. Fetuins are carrier proteins like albumin. Fetuin-A / AHSG forms soluble complexes with calcium and phosphate and thus is a carrier of otherwise insoluble calcium phosphate. Thus AHSG / Fetuin A is a potent inhibitor of pathological calcification. Fetuin A / FETUA promotes phagocytosis of apoptotic cells and plays a role in bone development, calcium homeostasis and insulin sensitivity. Mice deficient in Fetuin-A show systemic calcification of soft tissues.
Molecular Weight 38.2 kDa
NCBI Accession NP_001613
UniProt P02765
Research Area Serum/Plasma Proteins
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer PBS, pH 7.4
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C-8 °C), After reconstitution under sterile conditions for 1 month (4 °C-8 °C) or 3 months (-20 °C to -70 °C).
Supplier Images
SDS-PAGE (SDS) image for alpha-2-HS-Glycoprotein (AHSG) (AA 19-367) (Active) protein (His tag) (ABIN2181088) Human Fetuin A, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained...
Background publications Lee, Bongcam-Rudloff, Sollner, Jahnen-Dechent, Claesson-Welsh: "Type 3 cystatins; fetuins, kininogen and histidine-rich glycoprotein." in: Frontiers in bioscience (Landmark edition), Vol. 14, pp. 2911-22, 2009 (PubMed).

Heiss, Eckert, Aretz, Richtering, van Dorp, Schäfer, Jahnen-Dechent: "Hierarchical role of fetuin-A and acidic serum proteins in the formation and stabilization of calcium phosphate particles." in: The Journal of biological chemistry, Vol. 283, Issue 21, pp. 14815-25, 2008 (PubMed).

Jahnen-Dechent, Trindl, Godovac-Zimmermann, Müller-Esterl: "Posttranslational processing of human alpha 2-HS glycoprotein (human fetuin). Evidence for the production of a phosphorylated single-chain form by hepatoma cells." in: European journal of biochemistry / FEBS, Vol. 226, Issue 1, pp. 59-69, 1994 (PubMed).