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  • 产品名称：CD5Molecule(CD5)(AA25-372)(Active)protein(Histag)

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  • 产品厂商：ACROBiosystems
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简单介绍：
CD5Molecule(CD5)(AA25-372)(Active)protein(Histag)
详情介绍：
Characteristics This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 40.5 kDa. The protein migrates as 48-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Purity >95 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
Background T-cell surface glycoprotein CD5 is also known as Lymphocyte antigen T1/Leu-1 and LEU1,which is phosphorylated on tyrosine residues by LYN,so CD5 can create s for PTPN6/SHP-1.CD5 may act as a receptor in regulating T-cell proliferation. CD5 is expressed at various developmental and activation stages on human B cells.CD5 is a well established negative regulator of TCR and BCR signalling.CD5-positive cells may also prevent the emergence of autoimmunity by provision of cytokines such as IL-10. Development,selection and function of different B- and T-cell subsets or their preferential survival may be directly or indirectly dependent on different glycan structures associated with CD5 or CD5-like molecules.
Molecular Weight 40.5 kDa
NCBI Accession NP_055022
UniProt P06127
Research Area Stem Cells, Hematopoietic Progenitors, Adaptive Immunity, CD Antigens, Surface Receptors of Immune Cells
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer PBS, pH 7.4
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).
Supplier Images
SDS-PAGE (SDS) image for CD5 Molecule (CD5) (AA 25-372) (Active) protein (His tag) (ABIN2180809) Human CD5, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained over...
Background publications Rodamilans, Muñoz, Bragado-Nilsson, Sarrias, Padilla, Blanco, Lozano, Montoya: "Crystal structure of the third extracellular domain of CD5 reveals the fold of a group B scavenger cysteine-rich receptor domain." in: The Journal of biological chemistry, Vol. 282, Issue 17, pp. 12669-77, 2007 (PubMed).

Brill, Salomon, Ficarro, Mukherji, Stettler-Gill, Peters: "Robust phosphoproteomic profiling of tyrosine phosphorylation sites from human T cells using immobilized metal affinity chromatography and tandem mass spectrometry." in: Analytical chemistry, Vol. 76, Issue 10, pp. 2763-72, 2004 (PubMed).

Roebroek, van de Velde, Van Bokhoven, Broers, Ramaekers, Van de Ven: "Cloning and expression of alternative transcripts of a novel neuroendocrine-specific gene and identification of its 135-kDa translational product." in: The Journal of biological chemistry, Vol. 268, Issue 18, pp. 13439-47, 1993 (PubMed).

Van de Velde, von Hoegen, Luo, Parnes, Thielemans: "The B-cell surface protein CD72/Lyb-2 is the ligand for CD5." in: Nature, Vol. 351, Issue 6328, pp. 662-5, 1991 (PubMed).