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  • 产品名称：CarbonicAnhydraseII(CA2)(AA2-260)(Active)protein(Histag)

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  • 产品厂商：ACROBiosystems
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简单介绍：
CarbonicAnhydraseII(CA2)(AA2-260)(Active)protein(Histag)
详情介绍：
Characteristics This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 30 kDa. The protein migrates as 30 kDa under reducing (R) condition (SDS-PAGE).
Purity >95 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
ProductDetails: Biological Activity Comment Biological Activity: Measured by its esterase activity for digestion of 4-Nitrophenyl Acetate(4-NPA). The specific activity is > 150 pmoles/min/μg.
Background Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes. CAs form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons (or vice versa), a reversible reaction that occurs rather slowly in the absence of a catalyst. One of the functions of the enzyme in animals is to interconvert carbon dioxide and bicarbonate to maintain acid-base balance in blood and other tissues, and to help transport carbon dioxide out of tissues. The active site of most carbonic anhydrases contains a zinc ion. They are, therefore, classified as metalloenzymes. There are at least five distinct CA families (α, β, γ, δ and ε). These families have no significant amino acid sequence similarity and in most cases are thought to be an example of convergent evolution. The α-CAs are found in humans. Carbonic anhydrase II (CA2) is also known as Carbonate dehydratase II, Carbonic anhydrase C, is one of fourteen forms of human α carbonic anhydrases. Defects in this enzyme are associated with osteopetrosis and renal tubular acidosis. Renal carbonic anhydrase allows the reabsorption of sodium ions in the proximal tubule. Carbonic anhydrase II has been shown to interact with Band 3 and Sodium-hydrogen antiporter 1.
Molecular Weight 30 kDa
NCBI Accession NP_000058
UniProt P00918
Research Area Enzymes, Metabolism
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer 20 mM Tris, pH 8.0 with 150 mM NaCl, 1 mM DTT
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).
Supplier Images
SDS-PAGE (SDS) image for Carbonic Anhydrase II (CA2) (AA 2-260) (Active) protein (His tag) (ABIN2180674) Human Carbonic Anhydrase II, His Tag on SDS-PAGE under reducing (R) condition. The ge...
Background publications Yu, Price, Badger: "A Mutant Isolated from the Cyanobacterium Synechococcus PCC7942 Is Unable to Adapt to Low Inorganic Carbon Conditions." in: Plant physiology, Vol. 104, Issue 2, pp. 605-611, 2002 (PubMed).

Nair, Calderone, Christianson, Fierke: "Altering the mouth of a hydrophobic pocket. Structure and kinetics of human carbonic anhydrase II mutants at residue Val-121." in: The Journal of biological chemistry, Vol. 266, Issue 26, pp. 17320-5, 1991 (PubMed).