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  • 产品名称：SonicHedgehog(SHH)(AA24-197)(Active)protein(Histag)

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  • 产品厂商：ACROBiosystems
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简单介绍：
SonicHedgehog(SHH)(AA24-197)(Active)protein(Histag)
详情介绍：
Characteristics This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 20.4 kDa. The protein migrates as 22 kDa under reducing (R) condition (SDS-PAGE).
Purity >95 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
ProductDetails: Biological Activity Comment Biological Activity: Measured by its ability to induce alkaline phosphatase production by C3H10T1/2 mouse embryonic fibroblast cells. Nakamura, T. et al. (1997) Biochem. Biophys. Res. Commun.237:465. The ED50 for this effect is typically 1.5-10 μg/mL.
Background Sonic hedgehog protein (SHH) is also known as HHG-1, which belongs to the hedgehog family. SHH is expressed in fetal intestine, liver, lung, and kidney and not expressed in ***** tissues. Sonic hedgehog protein can be cleavage into sonic hedgehog protein N-product and sonic hedgehog protein C-product by autolysis. SHH binds to the patched (PTC) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes. In the absence of SHH, PTC represses the constitutive signaling activity of SMO. Also regulates another target, the gli oncogene. Intercellular signal essential for a variety of patterning events during development. The C-terminal domain displays an autoproteolysis activity and a cholesterol transferase activity. The N-product is the active species in both local and long-range signaling, whereas the C-product has no signaling activity.
Molecular Weight 20.4 kDa
NCBI Accession NP_000184
UniProt Q15465
Research Area Cancer, Organogenesis, Embryogenesis, Embryonic stem cells, Transcription Factors
Pathways Hedgehog Signaling, Dopaminergic Neurogenesis, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer PBS, pH 7.4
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C-8 °C), After reconstitution under sterile conditions for 1 month (4 °C-8 °C) or 3 months (-20 °C to -70 °C).
Supplier Images
SDS-PAGE (SDS) image for Sonic Hedgehog (SHH) (AA 24-197) (Active) protein (His tag) (ABIN2181760) Human Sonic Hedgehog, His Tag on SDS-PAGE under reducing (R) condition. The gel was s...
Background publications Pepinsky, Zeng, Wen, Rayhorn, Baker, Williams, Bixler, Ambrose, Garber, Miatkowski, Taylor, Wang, Galdes: "Identification of a palmitic acid-modified form of human Sonic hedgehog." in: The Journal of biological chemistry, Vol. 273, Issue 22, pp. 14037-45, 1998 (PubMed).

Marigo, Roberts, Lee, Tsukurov, Levi, Gastier, Epstein, Gilbert, Copeland, Seidman: "Cloning, expression, and chromosomal location of SHH and IHH: two human homologues of the Drosophila segment polarity gene hedgehog." in: Genomics, Vol. 28, Issue 1, pp. 44-51, 1995 (PubMed).