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  • 产品名称：TumorNecrosisFactorReceptorSuperfamily,Member11b(TNFRSF11B)(AA22-401)(Active)pr

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  • 产品厂商：ACROBiosystems
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简单介绍：
TumorNecrosisFactorReceptorSuperfamily,Member11b(TNFRSF11B)(AA22-401)(Active)protein(Histag)
详情介绍：
Characteristics This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 44.4 kDa. The protein migrates as 55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Purity >95 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
ProductDetails: Biological Activity Comment Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized rh TNFRSF11B at 10 μg/mL (100 μL/well) can bind human TNFSF11 Fc Chimera with a linear ranger of 4-256 ng/mL.
Background Tumor necrosis factor receptor superfamily member 11B (TNFRSF11B) is also known as Osteoclastogenesis inhibitory factor (OCIF), Osteoprotegerin (OPG). TNFRSF11B is a secreted homodimer protein, which can interact with TNFSF10 and TNFSF11. TNFRSF11B acts as decoy receptor for TNFSF11/RANKL and thereby neutralizes its function in osteoclastogenesis. TNFRSF11B inhibits the activation of osteoclasts and promotes osteoclast apoptosis in vitro. Bone homeostasis seems to depend on the local ratio between TNFSF11 and TNFRSF11B. TNFSF10/TRAIL binding blocks the inhibition of osteoclastogenesis.
Molecular Weight 44.4 kDa
NCBI Accession NP_002537
UniProt O00300
Research Area Cardiovascular, Atherosclerosis, Extracellular Matrix, Innate Immunity, Cytokines
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer PBS, pH 7.4
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).
Supplier Images
SDS-PAGE (SDS) image for Tumor Necrosis Factor Receptor Superfamily, Member 11b (TNFRSF11B) (AA 22-401) (Active) protein (His tag) (ABIN2181847) Human Osteoprotegerin, His Tag on SDS-PAGE under reducing (R) condition. The gel was ...
Background publications Luan, Lu, Jiang, Zhang, Wang, Yuan, Zhao, Wang, Wang: "Crystal structure of human RANKL complexed with its decoy receptor osteoprotegerin." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 189, Issue 1, pp. 245-52, 2012 (PubMed).

Prichett, Patton, Field, Brun, Emery, Tan, Rieman, McClung, Nadeau, Mooney, Suva, Gowen, Nuttall: "Identification and cloning of a human urea transporter HUT11, which is downregulated during adipogenesis of explant cultures of human bone." in: Journal of cellular biochemistry, Vol. 76, Issue 4, pp. 639-50, 2000 (PubMed).

Emery, McDonnell, Burke, Deen, Lyn, Silverman, Dul, Appelbaum, Eichman, DiPrinzio, Dodds, James, Rosenberg, Lee, Young: "Osteoprotegerin is a receptor for the cytotoxic ligand TRAIL." in: The Journal of biological chemistry, Vol. 273, Issue 23, pp. 14363-7, 1998 (PubMed).

Tsuda, Goto, Mochizuki, Yano, Kobayashi, Morinaga, Higashio: "Isolation of a novel cytokine from human fibroblasts that specifically inhibits osteoclastogenesis." in: Biochemical and biophysical research communications, Vol. 234, Issue 1, pp. 137-42, 1997 (PubMed).