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  • 产品名称：VascularEndothelialGrowthFactor(VEGF)(AA27-191)(Active)protein(Biotin)

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  • 产品厂商：ACROBiosystems
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简单介绍：
VascularEndothelialGrowthFactor(VEGF)(AA27-191)(Active)protein(Biotin)
详情介绍：
Brand MABSol®,UltraLys
Specificity The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with biotins using standard chemical labeling method. A standard biotin reagent (13.5 angstroms) is used in this product.
Characteristics The product does NOT contain any epitope tags. The protein has a calculated MW of 19 kDa (monomer). The protein migrates as 24 kDa (monomer) on a SDS-PAGE gel under reducing (R) condition due to glycosylation and 43-50 kDa under non-reducing (NR) condition.
Purity >95 % as determined by reduced SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
Grade Ultra Sensitive
ProductDetails: Biological Activity Comment Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized VEGFR2/R3-Fc at 5 μg/mL (100 μL/well) can bind Biotinylated rh VEGF165 (Catalog # VE5-H8210) with a linear range of 0.5-5 ng/mL.

SDS-PAGE: The primary amine of Biotinylated VEGF165 was labeled by biotin for detection in ELISA, dot blot or western blot using streptavidin or avidin-conjugated probes.
Background VEGF165 is the most abundant splice variant of VEGF-A. VEGF165 is produced by a number of cells including endothelial cells, macrophages and T cells. VEGF165 is involved in angiogenesis, vascular endothelial cell survival, growth, migration and vascular permeability. VEGF gene expression is induced by hypoxia, inflammatory cytokines and oncogenes. VEGF165 binds to heparan sulfate and is retained on the cell surface and in the extracellular matrix. VEGF165 binds to the receptor tyrosine kinases, VEGFR1 and VEGFR2. VEGF165 is the only splice variant that binds to co-receptors NRP-1 and NRP-2 that function to enhance VEGFR2 signaling. Binding of VEGF165 to VEGFR1 and VEGFR2 leads to activation of the PI3K/AKT, p38 MAPK, FAK and paxillin. VEGF plays a key role in tumor angiogenesis in many cancers.
Molecular Weight 19 kDa (monomer)
NCBI Accession NP_001020539
UniProt P15692
Research Area Signaling, Receptors, Immunology, Cytokines, Growth Factors, Inflammation
Comment

A chemically labeled biotinylated protein with ultra sensitivity.
The product is produced using a chemical labeling approach. The primary amines in the side chains of lysine residues and the N-terminus of protein are conjugated with biotins.
Chemical labeling usually results in multiple biotin attachment on a single protein molecule, which could potentially lead to higher detection sensitivity.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer PBS, pH 7.4
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).
Supplier Images
SDS-PAGE (SDS) image for Vascular Endothelial Growth Factor (VEGF) (AA 27-191) (Active) protein (Biotin) (ABIN2181906) Biotinylated Human VEGF165 on SDS-PAGE under reducing (R) and no-reducing (NR) condit...
Binding Studies (Bind) image for Vascular Endothelial Growth Factor (VEGF) (AA 27-191) (Active) protein (Biotin) (ABIN2181906) Immobilized human VEGFR2/R3, Fc tag at 2μg/mL (100 μL/well) can bind Biotinylated Hum...
Binding Studies (Bind) image for Vascular Endothelial Growth Factor (VEGF) (AA 27-191) (Active) protein (Biotin) (ABIN2181906) Immobilized Human Neuropilin-1, His Tag (Cat # NR1-H5228) at 2 μg/mL (100 μL/well) ca...
Background publications Takahashi, Shibuya: "The vascular endothelial growth factor (VEGF)/VEGF receptor system and its role under physiological and pathological conditions." in: Clinical science (London, England : 1979), Vol. 109, Issue 3, pp. 227-41, 2005 (PubMed).

Robinson, Stringer: "The splice variants of vascular endothelial growth factor (VEGF) and their receptors." in: Journal of cell science, Vol. 114, Issue Pt 5, pp. 853-65, 2001 (PubMed).

Neufeld, Cohen, Gengrinovitch, Poltorak: "Vascular endothelial growth factor (VEGF) and its receptors." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 13, Issue 1, pp. 9-22, 1999 (PubMed).