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  • 产品名称：CD3D&CD3E(AA22-105)(Active)protein(FcTag,DYKDDDDKTag,Histag)

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  • 产品厂商：ACROBiosystems
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简单介绍：
CD3D&CD3E(AA22-105)(Active)protein(FcTag,DYKDDDDKTag,Histag)
详情介绍：
Characteristics Human CD3 epsilon & CD3 delta Heterodimer Protein is produced by co-expression of CD3E and CD3D, has a calculated MW of 39.2 kDa (CD3E), 37.5 kDa (CD3D) . Subunit CD3E is fused with his-tagged human IgG1 Fc fragment at the C-terminus and subunit CD3D is fused with flag-tagged human IgG1 Fc fragment at the C-terminus. The predicted N-terminus is Asp 23 (CD3E) & Phe 22 (CD3D). The reducing (R) heterodimer protein migrates as 40-55 kDa due to glycosylation.
Purity >95 % as determined by SDS-PAGE.
Sterility 0.22 μm filtered
Endotoxin Level Less than 1.0 EU per μg by the LAL method.
ProductDetails: Biological Activity Comment Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized Human CD3E & CD3D heterodimer Fc Chimera (Catalog # CDD-H52W0) at 10 μg/mL (100 μL/well) can bind OKT3 mAb with a linear ranger of 3.4-50 ng/mL.
Sub Type Fusionprotein
Background T-cell surface glycoprotein CD3 delta & CD3 epsilon chain, also known as CD3D & CD3E or CD3D&CD3E respectively, are single-pass type I membrane proteins. CD3D, together with CD3- epsilon(CD3E) , CD3-gamma and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways.
Molecular Weight 39.2 kDa (CD3E), 37.5 kDa (CD3D)
Restrictions For Research Use only
Format Lyophilized
Reconstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer PBS, pH 7.4
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).
Supplier Images
SDS-PAGE (SDS) image for CD3D & CD3E (AA 22-105) (Active) protein (Fc Tag,DYKDDDDK Tag,His tag) (ABIN2180782) CD3D & CD3E (AA 22-105) (Active) protein (Fc Tag,DYKDDDDK Tag,His tag)
Background publications Gil, Busto, Garcillán, Chean, García-Rodríguez, Díaz-Alderete, Navarro, Reiné, Mencía, Gurbindo, Beléndez, Gordillo, Duchniewicz, Höhne, García-Sánchez, Fernández-Cruz, López-Granados, Schamel et al.: "A leaky mutation in CD3D differentially affects ?? and ?? T cells and leads to a T??-T??+B+NK+ human SCID. ..." in: The Journal of clinical investigation, Vol. 121, Issue 10, pp. 3872-6, 2011 (PubMed).

Arbuckle, Gold, Andres, Schwartzman, Chaikelson: "The role of psychosocial context, age, and intelligence in memory performance of older men." in: Psychology and aging, Vol. 7, Issue 1, pp. 25-36, 1992 (PubMed).

Clevers, Dunlap, Wileman, Terhorst: "Human CD3-epsilon gene contains three miniexons and is transcribed from a non-TATA promoter." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 85, Issue 21, pp. 8156-60, 1988 (PubMed).