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  • 产品名称：Goatanti-HumanIgG(F(ab')2Region)Antibody(PE)

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  • 产品厂商：Acris-antibodies
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简单介绍：
Goatanti-HumanIgG(F(ab')2Region)Antibody(PE)
详情介绍：
Immunogen Human IgG F(ab')2 fragment.
Purification Immunoaffinity chromatography.
This product was prepared from monospecific antiserum by Immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
Background Synonyms: Human Immunoglobulin G
Research Area Immunology, Secondary Antibodies
Application Notes Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as otherantibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. The maximum amount of reagent required to stain 1 x 10^6 cells in Flow cytometry isapproximately 1.0 g of antibody conjugate. Lesser amounts of reagent may be sufficientfor staining. As a general guideline dilutions of 1:100 to 1:250 should be suitable for most applications.

Other applications not tested.
Otimal dilutions are dependent on conditions and should be determined by the user.
Comment

Absorption / Emission: 490 nm, 545 nm and 565 nm / 580 nm
Restrictions For Research Use only
Format Liquid
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % (w/v) Sodium Azide as preservative and 10 mg/mL Bovine Serum Albumin (BSA), IgG and Protease free, as stabilizer.
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice DO NOT FREEZE! Store reagent in the dark. Use subdued lighting during handling and incubation of cells prior to analysis.
Dilute only prior to immediate use
Storage 4 °C
Storage Comment This antibody is stable at 2-8 °C as an undiluted liquid.