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产品名称:Goatanti-Mouse(Murine)IgG2bAntibody(HRP)
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产品厂商:Acris-antibodies
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简单介绍:
Goatanti-Mouse(Murine)IgG2bAntibody(HRP)
详情介绍:
Product details
Product details
Immunogen
Mouse IgG2b paraproteins
Isotype
IgG
No Cross-Reactivity
Human
Cross-Reactivity (Details)
Species reactivity (tested):Mouse.
Purification
Affinity Chromatography
Target details
Target details
Background
Immunoglobulin G (IgG), is one of the most abundant proteins in human serum with normal levels between 8-17 mg/mL in ***** blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions, to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 10e11 variants.Synonyms: Mouse Immunoglobulin G2b
Research Area
Secondary Antibodies
Application Details
Application Details
Application Notes
Immunohistochemistry on Frozen and Paraffin Sections. ELISA: 1/4000-1/8000. Western Blot.
Other applications not tested.
Otimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Handling
Handling
Format
Liquid
Buffer
PBS, 0.01 % Thiomersal, 50 % Glycerol
Preservative
Thimerosal (Merthiolate)
Precaution of Use
Biohazard Informations: This product contains thimerosal which is hazardous.
Handling Advice
Avoid repeated freezing and thawing. Dilute only prior to immediate use
Centrifuge product if not completely clear after standing at room temperature.
Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.
Storage
4 °C/-20 °C
Storage Comment
Store undiluted at 2-8 °C for one month or (in aliquots) at -28 °C for longer.
References
References
Background publications
Knipping, McNeal, Crienen, van Amerongen, Garssen, Vant Land: "A gastrointestinal rotavirus infection mouse model for immune modulation studies."
in:
Virology journal
, Vol. 8, pp. 109, 2011 (PubMed).