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  • 产品名称:anti-CD46Molecule,ComplementRegulatoryProtein(CD46)antibody(APC)

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  • 产品厂商:Acris-antibodies
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简单介绍:
anti-CD46Molecule,ComplementRegulatoryProtein(CD46)antibody(APC)
详情介绍:
Immunogen HPB-ALL human T cell line
Clone MEM-258
Isotype IgG1
Specificity This antibody recognizes an epitope on SCR4 (the membrane-proximal SCR) domain of CD46 (Membrane cofactor protein). CD46 is 56-66 kDa dimeric transmembrane protein expressed on T and B lymphocytes, platelets, monocytes, granulocytes, endothelial cells, epithelial cells and fibroblast, it is negative on erythrocytes.
Cross-Reactivity (Details) Species reactivity (tested):Human, bovine
Alternative Name CD46 / MCP (CD46 Antibody Abstract)
Background CD46 (MCP, membrane cofactor protein) is a multifunctional cell surface transmembrane protein that binds and inactivates C3b and C4b complement fragments, regulates T cell-induced inflammatory responses by either inhibiting (CD46-1 isoform) or increasing (CD46-2 isoform) the contact hypersensitivity reaction. CD46 also serves as a receptor for several human pathogens (both bacteria and viruses), and its ligation alteres T lymphocyte polarization toward antigen-presenting cells or target cells, inhibiting lymphocyte function. CD46 is a protector of placental tissue and is also expressed on the inner acrosomal membrane of spermatozoa.Synonyms: MIC10, Membrane cofactor protein, TLX, Trophoblast leukocyte common antigen
Gene ID 4179
NCBI Accession NP_002380
UniProt P15529
Research Area CD Antigens, Surface Receptors of Immune Cells
Pathways Regulation of Actin Filament Polymerization
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Buffer Phosphate buffered saline (PBS) containing 15 mM sodium azide and 0.2 % (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Dilute only prior to immediate use
Storage 4 °C
Storage Comment Store the antibody (undiluted) at 2-8 °C. DO NOT FREEZE! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Background publications Kälin, Amstutz, Gastaldelli, Wolfrum, Boucke, Havenga, DiGennaro, Liska, Hemmi, Greber: "Macropinocytotic uptake and infection of human epithelial cells with species B2 adenovirus type 35." in: Journal of virology, Vol. 84, Issue 10, pp. 5336-50, 2010 (PubMed).

Wang, Tuve, Erdman, Lieber: "Receptor usage of a newly emergent adenovirus type 14." in: Virology, Vol. 387, Issue 2, pp. 436-41, 2009 (PubMed).

Rebetz, Na, Su, Holmqvist, Edqvist, Nyberg, Widegren, Salford, Sjögren, Arnberg, Qian, Fan: "Fiber mediated receptor masking in non-infected bystander cells restricts adenovirus cell killing effect but promotes adenovirus host co-existence." in: PLoS ONE, Vol. 4, Issue 12, pp. e8484, 2009 (PubMed).

Hoffmann, Bayer, Heim, Potthoff, Nettelbeck, Wildner: "Evaluation of twenty-one human adenovirus types and one infectivity-enhanced adenovirus for the treatment of malignant melanoma." in: The Journal of investigative dermatology, Vol. 128, Issue 4, pp. 988-98, 2008 (PubMed).

Loré, Adams, Havenga, Precopio, Holterman, Goudsmit, Koup: "Myeloid and plasmacytoid dendritic cells are susceptible to recombinant adenovirus vectors and stimulate polyfunctional memory T cell responses." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 179, Issue 3, pp. 1721-9, 2007 (PubMed).

Fleischli, Sirena, Lesage, Havenga, Cattaneo, Greber, Hemmi: "Species B adenovirus serotypes 3, 7, 11 and 35 share similar s on the membrane cofactor protein CD46 receptor." in: The Journal of general virology, Vol. 88, Issue Pt 11, pp. 2925-34, 2007 (PubMed).

Weyand, Lee, Higashi, Cawley, Yoshihara, So: "Monoclonal antibody detection of CD46 clustering beneath Neisseria gonorrhoeae microcolonies." in: Infection and immunity, Vol. 74, Issue 4, pp. 2428-35, 2006 (PubMed).

Fremeaux-Bacchi, Moulton, Kavanagh, Dragon-Durey, Blouin, Caudy, Arzouk, Cleper, Francois, Guest, Pourrat, Seligman, Fridman, Loirat, Atkinson: "Genetic and functional analyses of membrane cofactor protein (CD46) mutations in atypical hemolytic uremic syndrome." in: Journal of the American Society of Nephrology : JASN, Vol. 17, Issue 7, pp. 2017-25, 2006 (PubMed).

Oliaro, Pasam, Waterhouse, Browne, Ludford-Menting, Trapani, Russell: "Ligation of the cell surface receptor, CD46, alters T cell polarity and response to antigen presentation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, Issue 49, pp. 18685-90, 2006 (PubMed).

Liszewski, Kemper, Price, Atkinson: "Emerging roles and new functions of CD46." in: Springer seminars in immunopathology, Vol. 27, Issue 3, pp. 345-58, 2005 (PubMed).

Fleischli, Verhaagh, Havenga, Sirena, Schaffner, Cattaneo, Greber, Hemmi: "The distal short consensus repeats 1 and 2 of the membrane cofactor protein CD46 and their distance from the cell membrane determine productive entry of species B adenovirus serotype 35." in: Journal of virology, Vol. 79, Issue 15, pp. 10013-22, 2005 (PubMed).

Gaggar, Shayakhmetov, Lieber: "CD46 is a cellular receptor for group B adenoviruses." in: Nature medicine, Vol. 9, Issue 11, pp. 1408-12, 2003 (PubMed).

Marie, Astier, Rivailler, Rabourdin-Combe, Wild, Horvat: "Linking innate and acquired immunity: divergent role of CD46 cytoplasmic domains in T cell induced inflammation." in: Nature immunology, Vol. 3, Issue 7, pp. 659-66, 2002 (PubMed).

Okada, Liszewski, Atkinson, Caparon: "Membrane cofactor protein (CD46) is a keratinocyte receptor for the M protein of the group A streptococcus." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 92, Issue 7, pp. 2489-93, 1995 (PubMed).