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  • 产品名称：anti-TAP1antibody(Transporter1,ATP-BindingCassette,Sub-FamilyB(MDR/TAP))(AA507-

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  • 产品厂商：Acris-antibodies
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简单介绍：
anti-TAP1antibody(Transporter1,ATP-BindingCassette,Sub-FamilyB(MDR/TAP))(AA507-748)(FITC)
详情介绍：
Immunogen Recombinant Human TAP1 (aa 507-748)
Clone TAP1-28
Isotype IgG1
Specificity This antibody reacts with TAP1 on Flow Cytometry.
Cross-Reactivity (Details) Species reactivity (tested):Human.
Characteristics Synonyms: PSF1, RING4, Y3, Antigen peptide transporter 1, ATP-binding cassette sub-family B member2, Peptide supply factor 1, Peptide transporter PSF1, Peptide transporter involved in antigenprocessing 1
Purification Protein-A Agarose Chromatography of hybridoma supernatant.
Alternative Name ABCB2 / APT1 / TAP1 (TAP1 Antibody Abstract)
Background The transporter associated with antigen processing (TAP) is a critical component of the antigen presentation pathway. Assembly of peptides with newly synthesized class I molecules is assisted by many proteins. These include calreticulin, ERp57 and tapasin. These components together with TAP and the MHC class I subunits, constitute a large complex called the "MHC class I peptide-loading complex". Upon binding to peptides, class I molecules are released from the assembly complex and transit to the cell surface for immune surveillance by T cells. Two structurally related subunits of the TAP transporter, TAP1 and TAP2, form a complex on the ER membrane that is necessary and sufficient for peptide translocation from the cytosol into the ER. TAP1-mediated ATP hydrolysis is not essential for peptide translocation but that TAP2-mediated ATP hydrolysis is critical, not only for translocation, but for peptide binding.Synonyms: ATP-binding cassette sub-family B member 2, Antigen peptide transporter 1, PSF1, Peptide supply factor 1, Peptide transporter PSF1, Peptide transporter involved in antigen processing 1, RING4, Y3
Gene ID 6890
UniProt Q03518
Pathways Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process
Application Notes Flow Cytometry: 10-20 μg/mL (final concentration). Positive Controls: LCL721, Jurkat. Detailed procedure is provided in Protocols.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Protocol Flow Cytometric analysisWe usually use Fisher tubes or equivalents as reaction tubes for all steps described below. 1) Wash the cells 3 times with washing buffer [PBS containing 2% fetal calf serum (FCS) and0. 1% NaN3]. 2) Add 200 µL of 4% paraformaldehyde (PFA)/PBS to the cell pellet after tapping. Mix well,then fix the cells for 15 minutes at 4°C. 3) Wash the cells 3 times with washing buffer. 4) Add 200 µL of 70% ethanol to the cell pellet after tapping. Mix well, then permeabilizethe cells for 30 minutes at -20°C. 5) Wash the cells 3 times with washing buffer. 6) Add 20 µL of Clear Back (human Fc receptor blocking reagent) to the cell pellet aftertapping. Mix well and incubate for 5 minutes at room temperature (20~25°C). 7) Add 30 µL of the primary antibody at the concentration as suggest in the APPLICATIONSdiluted in the washing buffer. Mix well and incubate for 30 minutes at room temperature. 8) Add 1 mL of the washing buffer followed by centrifugation at 500 x g for 1 minute at roomtemperature. Remove supernatant by careful aspiration. 9) Resuspend the cells with 500 µL of the washing buffer and analyze by a flow cytometer. Positive Controls: LCL721, Jurkat
Restrictions For Research Use only
Concentration 50 µg/mL
Buffer PBS containing 1 % BSA and 0.09 % Sodium Azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store the antibody undiluted at 2-8 °C.
Shelf life: one year from despatch.
Expiry Date 12 months
Supplier Images
Flow Cytometry (FACS) image for anti-TAP1 antibody (Transporter 1, ATP-Binding Cassette, Sub-Family B (MDR/TAP)) (AA 507-748) (FITC) (ABIN492591) anti-Transporter 1, ATP-Binding Cassette, Sub-Family B (MDR/TAP) (TAP1) (AA 507-748) antibody (FITC)