产品详情
所在位置: 首页> 产品目录> 一抗>
查看大图

  • 产品名称：anti-CD34antibody(CD34Molecule)(FITC)

  • 产品型号：
  • 产品厂商：Acris-antibodies
  • 产品文档：
你添加了1件商品 查看购物车
简单介绍：
anti-CD34antibody(CD34Molecule)(FITC)
详情介绍：
Immunogen Human endothelial vesicles
Clone QBEnd-10
Isotype IgG1
Specificity This antibody reacts with Class II epitope on CD34 (Mucosialin), a 110-115 kDa monomeric transmembrane phosphoglycoprotein expressed on hematopoietic progenitors cells and on the most pluripotential stem cells, it is gradually lost on progenitor cells. This antibody has been also used as an endothelial marker.
Cross-Reactivity (Details) Species reactivity (tested):Human, Non-Human Primates, negative on Rat, Bovine, Sheep, Canine (Dog)
Alternative Name CD34 (CD34 Antibody Abstract)
Background CD34 is a highly glycosylated monomeric 111-115 kDa surface protein, which is present on many stem cell populations. It is a well established stem cell marker, though its expression on human hematopoietic stem cells is reversible. CD34 probably serves as a surface receptor that undergoes receptor-mediated endocytosis and regulates adhesion, differentiation and proliferation of hematopoietic stem cells and other progenitors. CD34 expression is likely to represent a specific state of hematopoietic development that may have altered adhering properties with expanding and differentiating capabilities in both in vitro and in vivo conditions.Synonyms: Hematopoietic progenitor cell antigen CD34, Hematopoietic progenitor cell marker
Gene ID 947
NCBI Accession NP_001020280
UniProt P28906
Research Area Hematopoietic Progenitors, Hematopoietic Stem Cells, Cardiogenesis, Lineage Markers, CD Antigens, Surface Receptors of Immune Cells
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Buffer Phosphate buffered saline (PBS) containing 15 mM sodium azide and 0.2 % (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice DO NOT FREEZE! This product is photosensitive and should be protected from light.
Storage 4 °C
Background publications Gangenahalli, Singh, Verma, Gupta, Sharma, Chandra, Luthra: "Hematopoietic stem cell antigen CD34: role in adhesion or homing." in: Stem cells and development, Vol. 15, Issue 3, pp. 305-13, 2006 (PubMed).

Gangenahalli, Singh, Verma, Gupta, Sharma, Chandra, Gulati, Luthra: "Three-dimensional structure prediction of the interaction of CD34 with the SH3 domain of Crk-L." in: Stem cells and development, Vol. 14, Issue 5, pp. 470-7, 2005 (PubMed).

Krauter, Hartl, Hambach, Kohlenberg, Gunsilius, Ganser, Heil: "Receptor-mediated endocytosis of CD34 on hematopoietic cells after stimulation with the monoclonal antibody anti-HPCA-1." in: Journal of hematotherapy & stem cell research, Vol. 10, Issue 6, pp. 863-71, 2002 (PubMed).

Dao, Arevalo, Nolta: "Reversibility of CD34 expression on human hematopoietic stem cells that retain the capacity for secondary reconstitution." in: Blood, Vol. 101, Issue 1, pp. 112-8, 2002 (PubMed).

Poblet, Jimenez-Acosta, Rocamora: "QBEND/10 (anti-CD34 antibody) in external root sheath cells and follicular tumors." in: Journal of cutaneous pathology, Vol. 21, Issue 3, pp. 224-8, 1994 (PubMed).

Traoré, Hirn: "Certain anti-CD34 monoclonal antibodies induce homotypic adhesion of leukemic cell lines in a CD18-dependent and a CD18-independent way." in: European journal of immunology, Vol. 24, Issue 10, pp. 2304-11, 1994 (PubMed).

Grimsley, Amos, Gordon, Greaves: "Rapid positive selection of CD34+ cells using magnetic microspheres coated with monoclonal antibody QBEND/10 linked via a cleavable disulphide bond." in: Leukemia, Vol. 7, Issue 6, pp. 898-908, 1993 (PubMed).

Kuzu, Bicknell, Harris, Jones, Gatter, Mason: "Heterogeneity of vascular endothelial cells with relevance to diagnosis of vascular tumours." in: Journal of clinical pathology, Vol. 45, Issue 2, pp. 143-8, 1992 (PubMed).

Sutherland, Marsh, Davidson, Baker, Keating, Mellors: "Differential sensitivity of CD34 epitopes to cleavage by Pasteurella haemolytica glycoprotease: implications for purification of CD34-positive progenitor cells." in: Experimental hematology, Vol. 20, Issue 5, pp. 590-9, 1992 (PubMed).

Ramani, Bradley, Fletcher: "QBEND/10, a new monoclonal antibody to endothelium: assessment of its diagnostic utility in paraffin sections." in: Histopathology, Vol. 17, Issue 3, pp. 237-42, 1991 (PubMed).

Schlingemann, Rietveld, de Waal, Bradley, Skene, Davies, Greaves, Denekamp, Ruiter: "Leukocyte antigen CD34 is expressed by a subset of cultured endothelial cells and on endothelial abluminal microprocesses in the tumor stroma." in: Laboratory investigation; a journal of technical methods and pathology, Vol. 62, Issue 6, pp. 690-6, 1990 (PubMed).