产品详情
所在位置: 首页> 产品目录> 一抗>
查看大图

  • 产品名称：anti-C5AR1antibody(ComplementComponent5aReceptor1)(PE)

  • 产品型号：
  • 产品厂商：Acris-antibodies
  • 产品文档：
你添加了1件商品 查看购物车
简单介绍：
anti-C5AR1antibody(ComplementComponent5aReceptor1)(PE)
详情介绍：
Immunogen RBL-2H3 (rat basophilic leukemia) transfected cells expressing human C5aR on the cell surface Donor: Lou/c rat spleen cells
Clone 8D6
Isotype IgG2a
Specificity This monoclonal antibody detects an epitope on the N terminus (aa 1-15) of the human C5a receptor (also known as CD88) found on human myeloid cells including granulocytes and monocytes/macrophages. It does not bind to tubular epithelial cells in situ under normal or inflammatory conditions. C5aR binds to C5a, a 74 aa peptide cleaved from complement protein C5. C5a receptor has seven transmembrane segments and is a member of the rhodopsin subfamily of G protein coupled receptors.
Purification Affinity chromatography on Protein G
Alternative Name CD88 / C5R1 (C5AR1 Antibody Abstract)
Background Synonyms: C5AR1, C5a anaphylatoxin chemotactic receptor, C5a-R, C5aR, Complement Component 5a Receptor 1
Gene ID 728
NCBI Accession NP_001727
UniProt P21730
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Concentration 0,1 mg/mL
Buffer PBS containing 0.02 % sodium azide (NaN3) as preservative and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/mL.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice DO NOT FREEZE!
Storage 4 °C
Storage Comment Store the antibody undiluted at 2-8 °C.
Background publications Kiafard, Tschernig, Schweyer, Bley, Neumann, Zwirner: "Use of monoclonal antibodies to assess expression of anaphylatoxin receptors in tubular epithelial cells of human, murine and rat kidneys." in: Immunobiology, Vol. 212, Issue 2, pp. 129-39, 2007 (PubMed).

Weinmann, Gutzmer, Zwirner, Wittmann, Langer, Lisewski, Mommert, Kapp, Werfel: "Up-regulation of C5a receptor expression and function on human monocyte derived dendritic cells by prostaglandin E2." in: Immunology, Vol. 110, Issue 4, pp. 458-65, 2003 (PubMed).

Zwirner, Fayyazi, Götze: "Expression of the anaphylatoxin C5a receptor in non-myeloid cells." in: Molecular immunology, Vol. 36, Issue 13-14, pp. 877-84, 2000 (PubMed).

Fayyazi, Sandau, Duong, Götze, Radzun, Schweyer, Soruri, Zwirner: "C5a receptor and interleukin-6 are expressed in tissue macrophages and stimulated keratinocytes but not in pulmonary and intestinal epithelial cells." in: The American journal of pathology, Vol. 154, Issue 2, pp. 495-501, 1999 (PubMed).

Werfel, Oppermann, Begemann, Götze, Zwirner: "C5a receptors are detectable on mast cells in normal human skin and in psoriatic plaques but not in weal and flare reactions or in uticaria pigmentosa by immunohistochemistry." in: Archives of dermatological research, Vol. 289, Issue 2, pp. 83-6, 1997 (PubMed).

Werfel, Zwirner, Oppermann, Sieber, Begemann, Drommer, Kapp, Götze: "CD88 antibodies specifically bind to C5aR on dermal CD117+ and CD14+ cells and react with a desmosomal antigen in human skin." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 157, Issue 4, pp. 1729-35, 1996 (PubMed).