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  • 产品名称：anti-CD4antibody(CD4Molecule)(Domain1)(FITC)

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  • 产品厂商：Acris-antibodies
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简单介绍：
anti-CD4antibody(CD4Molecule)(Domain1)(FITC)
详情介绍：
Immunogen Rat thymocyte membrane preperation.
Clone W3-25
Isotype IgG1
Purification Affinity Chromatography on Protein G.
Alternative Name CD4 (CD4 Antibody Abstract)
Background CD4 is a single chain transmembraneous glycoprotein (59 kDa) which belongs to the immunoglobulin superfamily. CD4 is present on a subset of T lymphocytes ("helper/inducer" T cells) and is also expressed at a lower level on monocytes, tissue macrophages and granulocytes. The antigen is involved in binding to MHC class II molecules. The intracellular domain of the antigen is associated with p56lck protein tyrosine kinase.Synonyms: T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4
Gene ID 10116
UniProt P05540
Research Area Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells
Pathways TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events
Application Notes Flow cytometry: Use 10 μL of neat-1/10 diluted antibody to label 10^6 cells in 100 μL. Immunohistochemistry on Frozen and Paraffin Embedded Sections. This clone has been described reacting with paraffin-embedded material following PLP fixation (periodate-lysine- paraformaldehyde) (see Ref. 3).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 0.1 mg/mL
Buffer PBS, pH 7.4 with 0.09 % Sodium Azide as preservative and 1 % BSA as stabilizer.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Background publications Hofmann, Lachnit, Streppel, Witter, Neiss, Guntinas-Lichius, Angelov: "Increased expression of ICAM-1, VCAM-1, MCP-1, and MIP-1 alpha by spinal perivascular macrophages during experimental allergic encephalomyelitis in rats." in: BMC immunology, Vol. 3, pp. 11, 2003 (PubMed).

Whiteland, Nicholls, Shimeld, Easty, Williams, Hill: "Immunohistochemical detection of T-cell subsets and other leukocytes in paraffin-embedded rat and mouse tissues with monoclonal antibodies." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 43, Issue 3, pp. 313-20, 1995 (PubMed).

Pelegrí, Franch, Castellote, Castell: "Immunohistochemical changes in synovial tissue during the course of adjuvant arthritis." in: The Journal of rheumatology, Vol. 22, Issue 1, pp. 124-32, 1995 (PubMed).

Barclay: "The localization of populations of lymphocytes defined by monoclonal antibodies in rat lymphoid tissues." in: Immunology, Vol. 42, Issue 4, pp. 593-600, 1981 (PubMed).

Williams, Galfrè, Milstein: "Analysis of cell surfaces by xenogeneic myeloma-hybrid antibodies: differentiation antigens of rat lymphocytes." in: Cell, Vol. 12, Issue 3, pp. 663-73, 1978 (PubMed).