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  • 产品名称：anti-CD80antibody(CD80)(PE)

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简单介绍：
anti-CD80antibody(CD80)(PE)
详情介绍：
Immunogen BCL1 cells expressing CD80.
Clone RM80
Isotype IgG2a
Specificity This antibody recognises mouse CD80 (B7-1), a 60 kDa cell surface glycoprotein which is a member of the CD28/B7 family. In mice, CD80 is expressed on monocytes, peritoneal macrophages and dendritic cells, and expression may be significantly increased upon B lymphocytes by LPS and by IL-4.
Characteristics Synonyms: CD28LG, CD28LG1, LAB7, BB1, Activation B7-1 antigen, B7.1, T-lymphocyte activationantigen CD80, CTLA-4 counter-receptor B7.1
Purification Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Alternative Name CD80 (CD80 Antibody Abstract)
Background CD80 is a member of the Ig superfamily and serves as the ligand for two T cell molecules, CD28 and CTLA4. Interactions between CD28 and CD80 on activated B cells result in enhanced T cell activation. CD80 is rapidly induced on the surface of in vitro activated B cells, Epstein Barr Virus (EBV) transformed B cell lines, Burkitts lymphoma cell lines, freshly isolated follicular B lymphoma cells, T cells, and monocytes. It is also expressed at high levels in dendritic cells. It reacts weakly with a small proportion of non activated normal B cells and with HTLV1 infected T cells. CD80 does not react with peripheral monocytes, resting and activated normal T cells, T cell lines and T cell clones, nor with myelomonocytic cell lines.Synonyms: Activation B7-1 antigen, B7-1, B7.1, BB1, CD28LG, CD28LG1, CTLA-4 counter-receptor B7.1, LAB7, T-lymphocyte activation antigen CD80
Gene ID 12519
UniProt Q00609
Research Area Stem Cells, Hematopoietic Progenitors, Adaptive Immunity, CD Antigens, Surface Receptors of Immune Cells
Pathways TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Immune Effector Process
Application Notes Flow Cytometry: Use 10 μL of the suggested working dilution to label 1x10^6 cells in 100 μL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Reconstitution Reconstitute with 1.0 mL distilled water.
Concentration 0.1 mg/mL
Buffer PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative and 1 % BSA + 5 % sucrose as stabilizer.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store the antibody undiluted at 2-8 °C. DO NOT FREEZE! This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf life: One year from despatch.
Expiry Date 12 months
Background publications Nozawa, Ohata, Sakurai, Hashimoto, Miyajima, Yagita, Okumura, Azuma et al.: "Preferential blockade of CD8(+) T cell responses by administration of anti-CD137 ligand monoclonal antibody results in differential effect on development of murine acute and chronic graft-versus-host ..." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 167, Issue 9, pp. 4981-6, 2001 (PubMed).

Nakajima, Watanabe, Yoshino, Yagita, Okumura, Azuma: "Requirement of CD28-CD86 co-stimulation in the interaction between antigen-primed T helper type 2 and B cells." in: International immunology, Vol. 9, Issue 5, pp. 637-44, 1997 (PubMed).