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  • 产品名称:anti-CD90antibody(THY1)(PE)

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简单介绍:
anti-CD90antibody(THY1)(PE)
详情介绍:
Immunogen Purified Human brain Thy-1. Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS-1 myeloma cell line.
Clone F15-42-1
Isotype IgG1
Specificity SM1170R recognises the Human CD90 cell surface antigen. Reacts with Cynomolgus Monkey.
Purification Ion Exchange Chromatography.
Alternative Name CD90 (THY1 Antibody Abstract)
Background CD90 antigen is a 25 kD glycoprotein homologous to rat Thy-1. The antigen is expressed by a subset of CD34+ve cells in the bone marrow and by some cells within the thymus. CD90 is also expressed extensively within the brain.Synonyms: CDw90, THY1, Thy-1, Thy-1 membrane glycoprotein
Gene ID 7070
NCBI Accession NP_006279
UniProt P04216
Research Area CD Antigens, Surface Receptors of Immune Cells, Experimental Models
Pathways Cell-Cell Junction Organization
Application Notes Flow Cytometry: Use 10 μL of neat antibody to label 10^6 cells in 100 μL. This product is routinely tested in flow cytometry on the MOLT4 cell line.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Reconstitution Restore in 0.25 mL distilled water. Note. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. We recommend that the vial is gently vortexed after reconstitution and microcentrifuged before use.
Buffer PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative and 1 % BSA, 5 % Sucrose as stabilizer.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Prior to and following reconstitution store the antibody at 2-8 °C. DO NOT FREEZE!
Background publications Diaz-Romero, Nesic, Grogan, Heini, Mainil-Varlet: "Immunophenotypic changes of human articular chondrocytes during monolayer culture reflect bona fide dedifferentiation rather than amplification of progenitor cells." in: Journal of cellular physiology, Vol. 214, Issue 1, pp. 75-83, 2007 (PubMed).

Hagood, Prabhakaran, Kumbla, Salazar, MacEwen, Barker, Ortiz, Schoeb, Siegal, Alexander, Pardo, Selman: "Loss of fibroblast Thy-1 expression correlates with lung fibrogenesis." in: The American journal of pathology, Vol. 167, Issue 2, pp. 365-79, 2005 (PubMed).

Fiegel, Glüer, Roth, Rischewski, von Schweinitz, Ure, Lambrecht, Kluth: "Stem-like cells in human hepatoblastoma." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 52, Issue 11, pp. 1495-501, 2004 (PubMed).

Yoshino, Ami, Terao, Tashiro, Honda: "Upgrading of flow cytometric analysis for absolute counts, cytokines and other antigenic molecules of cynomolgus monkeys (Macaca fascicularis) by using anti-human cross-reactive antibodies." in: Experimental animals / Japanese Association for Laboratory Animal Science, Vol. 49, Issue 2, pp. 97-110, 2000 (PubMed).

McKenzie, Fabre: "Human thy-1: unusual localization and possible functional significance in lymphoid tissues." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 126, Issue 3, pp. 843-50, 1981 (PubMed).

Daar, Fabre: "Demonstration with monoclonal antibodies of an unusual mononuclear cell infiltrate and loss of normal epithelial membrane antigens in human breast carcinomas." in: Lancet, Vol. 2, Issue 8244, pp. 434-8, 1981 (PubMed).