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  • 产品名称：anti-KLRB1antibody(KillerCellLectin-LikeReceptorSubfamilyB,Member1)(Biotin)

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  • 产品厂商：Acris-antibodies
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简单介绍：
anti-KLRB1antibody(KillerCellLectin-LikeReceptorSubfamilyB,Member1)(Biotin)
详情介绍：
Immunogen Spleen and bone marrow cells from CE mice. Spleen cells from immunised (C3H x BALB/c) FI Hybrid were fused with cells of the Sp2/0 - Ag14 myeloma cell line.
Clone PK136
Isotype IgG2a
Specificity This antibody (clone PK136) recognises the NK1.1 cell surface antigen. Although previously thought to recognise only CD161c, recent data has shown that the PK136 antibody may also react with CD161b. CD161c expression itself is strain specific in mice, but recognition of CD161b by PK136 appears to be even more complex, as only some CD161b positive strains are labelled by the antibody. Engagement of CD161c has been reported to have activating function in NK cells, whilst engagement of CD161b is inhibitory. This antibody is useful for the identification of NK cells in selected strains of mice (positive on C57BL, FVB/N and NZB, but negative on AKR and BALB/c) and is also expressed by rare subsets of T cells and monocytes. The antibody has also been used for in vivo depletion of NK cells and in vitro activation of NK cells.
Purification Affinity Chromatography on Protein A
Alternative Name CD161 / KLRB1 (KLRB1 Antibody Abstract)
Background NK1.1 is a cell surface glycoprotein encoded by members of the NKR-P1 gene family. In the mouse the NKR-P1 family has three members, NKR-P1A, -B and -C, whilst in the human only one member has been identified. The human protein has received the designation CD161, and the mouse proteins have been referred to as CD161a, -b, -c etc.Synonyms: C-type lectin domain family 5 member B, CLEC5B, HNKR-P1a, Killer cell lectin-like receptor subfamily B member 1, NKRP1A, Natural killer cell surface protein P1A
Gene ID 3820
NCBI Accession NP_002249
UniProt Q12918
Research Area CD Antigens, Surface Receptors of Immune Cells
Application Notes Flow Cytometry: The Fc region of monoclonal antibodies may bind non-specifically to cellsexpressing low affinity fc receptors.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 0.1 mg/mL
Buffer PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative and 1 % BSA as stabilizer.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Background publications Kung, Su, Shannon, Miller: "The NKR-P1B gene product is an inhibitory receptor on SJL/J NK cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 162, Issue 10, pp. 5876-87, 1999 (PubMed).

Carlyle, Martin, Mehra, Attisano, Tsui, Zúñiga-Pflücker: "Mouse NKR-P1B, a novel NK1.1 antigen with inhibitory function." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 162, Issue 10, pp. 5917-23, 1999 (PubMed).

Arase, Arase, Park, Ohno, Ra, Saito: "Association with FcRgamma is essential for activation signal through NKR-P1 (CD161) in natural killer (NK) cells and NK1.1+ T cells." in: The Journal of experimental medicine, Vol. 186, Issue 12, pp. 1957-63, 1998 (PubMed).

Kung, Miller: "The NK1.1 antigen in NK-mediated F1 antiparent killing in vitro." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 154, Issue 4, pp. 1624-33, 1995 (PubMed).

Matera, Weiner, Schmid: "Structure and evolution of the U2 small nuclear RNA multigene family in primates: gene amplification under natural selection?" in: Molecular and cellular biology, Vol. 10, Issue 11, pp. 5876-82, 1990 (PubMed).

Koo, Dumont, Tutt, Hackett, Kumar: "The NK-1.1(-) mouse: a model to study differentiation of murine NK cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 137, Issue 12, pp. 3742-7, 1987 (PubMed).

Koo, Peppard: "Establishment of monoclonal anti-Nk-1.1 antibody." in: Hybridoma, Vol. 3, Issue 3, pp. 301-3, 1985 (PubMed).