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  • 产品名称：anti-CD3antibody(CD3)(PE)

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  • 产品厂商：Acris-antibodies
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简单介绍：
anti-CD3antibody(CD3)(PE)
详情介绍：
Immunogen Spleen cells from immunised SD rats were fused with cells of the NSO mouse myeloma cell line.
Clone KT3
Isotype IgG2a
Specificity This antibody recognises the CD3 antigen which is expressed by mature T cells.
Purification Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Alternative Name CD3 (CD3 Antibody Abstract)
Background Cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits: CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82 % of normal peripheral blood lymphocytes, 65-85 % of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.Synonyms: T-cell surface antigen T3/Leu-4, T-cell surface glycoprotein CD3, T3/Leu-4
Gene ID 12501
NCBI Accession NP_031674
UniProt P22646
Research Area Immunology, Adaptive Immunity, Hematopoietic Progenitors, Hematopoietic Stem Cells, CD Antigens, Surface Receptors of Immune Cells
Application Notes Flow Cytometry: use 10 μL of neat-1/10 diluted antibody to label 10^6 cells. For optimalstaining incubations should be performed at room temperature, The Fc region ofmonoclonal antibodies may bind non-specifically to cells expressing low affinity Fcreceptors.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Reconstitution Restore with 5.0 mL distilled water.
Buffer PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative and 1 % BSA as stabilizer.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Dilute only prior to immediate use
Storage 4 °C
Storage Comment Store the antibody undiluted at 2-8 °C. DO NOT FREEZE! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Background publications Lacroix-Lamandé, Mancassola, Naciri, Laurent: "Role of gamma interferon in chemokine expression in the ileum of mice and in a murine intestinal epithelial cell line after Cryptosporidium parvum infection." in: Infection and immunity, Vol. 70, Issue 4, pp. 2090-9, 2002 (PubMed).

Tomonari, Lovering: "T-cell receptor-specific monoclonal antibodies against a V beta 11-positive mouse T-cell clone." in: Immunogenetics, Vol. 28, Issue 6, pp. 445-51, 1988 (PubMed).