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  • 产品名称：anti-CD3antibody(CD3)(FITC)

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  • 产品厂商：Acris-antibodies
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简单介绍：
anti-CD3antibody(CD3)(FITC)
详情介绍：
Clone CA17-2A12
Isotype IgG1
Specificity This antibody recognises the canine CD3 cell surface antigen, expressed by mature T lymphocytes. This antibody should not be used with AM05866RP-N (mouse anti canine B-cells), in dual colour flow cytometry, due to non-specific interactions between the two reagents.
Characteristics Synonyms: T3/Leu-4, T-cell surface glycoprotein CD3, T-cell surface antigen T3/Leu-4
Purification Purified IgG prepared by affinity chromatography on Protein G
Alternative Name CD3 (CD3 Antibody Abstract)
Background T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits: CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82 % of normal peripheral blood lymphocytes, 65-85 % of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.Synonyms: T-cell surface antigen T3/Leu-4, T-cell surface glycoprotein CD3, T3/Leu-4
Gene ID 442981
UniProt P27597
Research Area Immunology, Adaptive Immunity, Hematopoietic Progenitors, Hematopoietic Stem Cells, CD Antigens, Surface Receptors of Immune Cells
Application Notes Flow Cytometry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 0.1 mg/mL
Buffer Phosphate buffered saline pH 7.4 containing 0.09 % Sodium Azide, 1 % Bovine Serum Albumin
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.
Shelf life: one year from despatch.
Expiry Date 12 months
Supplier Images
Flow Cytometry (FACS) image for anti-CD3 antibody (CD3) (FITC) (ABIN316750) anti-CD3 (CD3) antibody (FITC)
Background publications McDonough, Moore: "Clinical, hematologic, and immunophenotypic characterization of canine large granular lymphocytosis." in: Veterinary pathology, Vol. 37, Issue 6, pp. 637-46, 2001 (PubMed).

Byrne, Kim, Chew, Reinhart, Hayek: "A standardized gating technique for the generation of flow cytometry data for normal canine and normal feline blood lymphocytes." in: Veterinary immunology and immunopathology, Vol. 73, Issue 2, pp. 167-82, 2000 (PubMed).

Vernau, Moore: "An immunophenotypic study of canine leukemias and preliminary assessment of clonality by polymerase chain reaction." in: Veterinary immunology and immunopathology, Vol. 69, Issue 2-4, pp. 145-64, 1999 (PubMed).

Moreno, Nieto, Chamizo, González, Blanco, Barker, Alva: "The immune response and PBMC subsets in canine visceral leishmaniasis before, and after, chemotherapy." in: Veterinary immunology and immunopathology, Vol. 71, Issue 3-4, pp. 181-95, 1999 (PubMed).