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  • 产品名称：anti-CD68antibody(CD68Molecule)(Biotin)

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  • 产品厂商：Acris-antibodies
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简单介绍：
anti-CD68antibody(CD68Molecule)(Biotin)
详情介绍：
Immunogen Rat spleen cells
Clone ED1
Isotype IgG1
Specificity CD68 antibody (Cat#BM4000B) recognises a single chain glycoprotein of 110 kDa that is expressed predominantly on the lysomal membrane of myeloid cells. Weak cell surface expression also occurs. The antigen is expressed by the majority of tissue macrophages, and weakly be peripheral blood granulocytes. Studies have shown that the antigen recognised by ED1 is the rat homologue of human CD68.
Purification Affinity Chromatography on Protein A
Alternative Name CD68 (CD68 Antibody Abstract)
Background The CD68 antigen is a 37kD transmembrane protein that is post-translationally glycosylated to give a protein of 87-115kD. CD68 is specifically expressed by tissue macrophages, Langerhans cells and at low levels by dendritic cells. It could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. It binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin bearing substrates or other cells.Synonyms: Gp110, Macrophage marker, Macrosialin
Gene ID 287435
NCBI Accession NP_001026808
UniProt Q4FZY1
Research Area Immunology, CD Antigens, Surface Receptors of Immune Cells
Application Notes Flow Cytometry: Use 10 mLof neat antibody to label 10^6 cells in 100 μL. (Results maybe enhanced using membrane permeabilisation).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 0.1 mg/mL
Buffer PBS, pH 7.2 with 0.09 % Sodium Azide as preservative and 1 % BSA as stabiliser
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Product cited in: Schneider, Meusel, Renker, Bauer, Holzinger, Roeder, Wanner, Gekle, Sauvant: "Low-dose indomethacin after ischemic acute kidney injury prevents downregulation of Oat1/3 and improves renal outcome." in: American journal of physiology. Renal physiology, Vol. 297, Issue 6, pp. F1614-21, 2009 (PubMed).

Background publications Damoiseaux, Döpp, Calame, Chao, MacPherson, Dijkstra: "Rat macrophage lysosomal membrane antigen recognized by monoclonal antibody ED1." in: Immunology, Vol. 83, Issue 1, pp. 140-7, 1995 (PubMed).

Bauer, Sminia, Wouterlood, Dijkstra: "Phagocytic activity of macrophages and microglial cells during the course of acute and chronic relapsing experimental autoimmune encephalomyelitis." in: Journal of neuroscience research, Vol. 38, Issue 4, pp. 365-75, 1994 (PubMed).

Dijkstra, Döpp, Joling, Kraal: "The heterogeneity of mononuclear phagocytes in lymphoid organs: distinct macrophage subpopulations in the rat recognized by monoclonal antibodies ED1, ED2 and ED3." in: Immunology, Vol. 54, Issue 3, pp. 589-99, 1985 (PubMed).