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  • 产品名称：anti-CD25antibody(Interleukin2Receptor,alpha)(FITC)

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  • 产品厂商：Acris-antibodies
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简单介绍：
anti-CD25antibody(Interleukin2Receptor,alpha)(FITC)
详情介绍：
Immunogen Stimulated Rat T cells. Spleen cells from immunised BALB/c mice were fused with cells of the NSO/1 mouse myeloma cell line.
Clone OX-39
Isotype IgG1
Specificity This antibody recognizes the alpha chain of CD25, otherwise known as IL-2 receptor alpha. This clone has been described reacting with paraffin-embedded material following PLP fixation (periodatelysine-paraformaldehyde). Clone OX-39 has been shown to weakly inhibit the binding of IL-2 to Con-A stimulated spleen blasts. We recommend the use of SM265LE for this purpose.
Purification Affinity chromatography on Protein G
Alternative Name CD25 / IL2RA (IL2RA Antibody Abstract)
Background CD25 is a 55 kDa type I membrane glycoprotein, expressed by activated T cells but not resting lymphocytes. CD25 is also expressed by dendritic cells found in the thymus medulla.Synonyms: IL-2 receptor alpha subunit, IL-2-RA, IL2-RA, Interleukin-2 receptor alpha chain, TAC antigen, p55
Gene ID 25704
NCBI Accession NP_037295
UniProt P26897
Research Area CD Antigens, Surface Receptors of Immune Cells, Cytokines, Receptors, Inflammation
Pathways JAK-STAT Signaling, Growth Factor Binding, Activated T Cell Proliferation
Application Notes Flow Cytometry: 1/10 - 1/20, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 0.5 mg/mL
Buffer PBS, pH 7.4 containing 0.09 % Sodium Azide and 1 % Bovine Serum Albumin
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Background publications Paterson, Jefferies, Green, Brandon, Corthesy, Puklavec, Williams: "Antigens of activated rat T lymphocytes including a molecule of 50,000 Mr detected only on CD4 positive T blasts." in: Molecular immunology, Vol. 24, Issue 12, pp. 1281-90, 1988 (PubMed).

Hayosh, Swanborg: "Autoimmune effector cells. IX. Inhibition of adoptive transfer of autoimmune encephalomyelitis with a monoclonal antibody specific for interleukin 2 receptors." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 138, Issue 11, pp. 3771-5, 1987 (PubMed).

Tellides, Dallman, Kupiec-Weglinski, Diamantstein, Morris: "Functional blocking of the interleukin-2 receptor (IL-2R) may be important in the efficacy of IL-2R antibody therapy." in: Transplantation proceedings, Vol. 19, Issue 5, pp. 4231-3, 1987 (PubMed).