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  • 产品名称：anti-ICAM-1antibody(IntercellularAdhesionMolecule1)(FITC)

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  • 产品厂商：Acris-antibodies
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简单介绍：
anti-ICAM-1antibody(IntercellularAdhesionMolecule1)(FITC)
详情介绍：
Immunogen Rat Ax cells (a HEV derived cell line). Remarks: Spleen cells from immunised BALB/c mice were fused with cells from the PAI mouse myeloma cell line.
Clone 1A29
Isotype IgG1
Specificity This antibody recognises the CD54 cell surface antigen, also known as intercellular adhesion molecule-1 (ICAM-1). Recent studies suggest that cross-linking of ICAM-1 using clone 1A29 induces calcium signalling. Functionally 1A29 inhibits homotypic aggregation of PHA blasts. We recommend the use of SM286LE for use in functional studies.
Cross-Reactivity (Details) Species reactivity (tested):Rat.
Purification Affinity Chromatography on Protein G
Alternative Name CD54 / ICAM1 (ICAM1 Antibody Abstract)
Background CD54 is a 90kD adhesion molecule belonging to the immunoglobulin superfamily. CD54 is a cell surface ligand of the lymphocyte integrin, LFA-1 and is known to play an important role in various cell-cell interactions in the immune system.Synonyms: ICAM-1, Intercellular adhesion molecule 1, Major Group Rhinovirus Receptor
Gene ID 25464
NCBI Accession NP_037099
UniProt Q00238
Pathways Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Regulation of Leukocyte Mediated Immunity, Thromboxane A2 Receptor Signaling
Application Notes Flow Cytometry: 1/25 - 1/100, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 0.5 mg/mL
Buffer PBS, pH 7.2, containing 1 % BSA as stabiliser and 0.09 % Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Background publications Neto, Oliveira: "Nanoscillator and gun under temperature effect." in: Journal of nanoscience and nanotechnology, Vol. 10, Issue 9, pp. 5755-8, 2010 (PubMed).

Trinh, Brignole-Baudouin, Labbé, Raphaël, Bourges, Baudouin: "The corneal endothelium in an endotoxin-induced uveitis model: correlation between in vivo confocal microscopy and immunohistochemistry." in: Molecular vision, Vol. 14, pp. 1149-56, 2008 (PubMed).

Banerjee, Figueiredo, Easty, Dick, Nicholls: "Development of organised conjunctival leucocyte aggregates after corneal transplantation in rats." in: The British journal of ophthalmology, Vol. 87, Issue 12, pp. 1515-22, 2003 (PubMed).

McKechnie, Gürr, Yamada, Copland, Braun: "Antigenic mimicry: Onchocerca volvulus antigen-specific T cells and ocular inflammation." in: Investigative ophthalmology & visual science, Vol. 43, Issue 2, pp. 411-8, 2002 (PubMed).

Kielian, Hickey: "Proinflammatory cytokine, chemokine, and cellular adhesion molecule expression during the acute phase of experimental brain abscess development." in: The American journal of pathology, Vol. 157, Issue 2, pp. 647-58, 2000 (PubMed).

Etienne, Adamson, Greenwood, Strosberg, Cazaubon, Couraud: "ICAM-1 signaling pathways associated with Rho activation in microvascular brain endothelial cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 161, Issue 10, pp. 5755-61, 1998 (PubMed).

Farwell, Roberts, Rabinowitz: "The effect of ribosomal protein S1 from Escherichia coli and Micrococcus luteus on protein synthesis in vitro by E. coli and Bacillus subtilis." in: Molecular microbiology, Vol. 6, Issue 22, pp. 3375-83, 1993 (PubMed).

Tamatani, Miyasaka et al.: "Identification of monoclonal antibodies reactive with the rat homolog of ICAM-1, and evidence for a differential involvement of ICAM-1 in the adherence of resting versus activated lymphocytes to high ..." in: International immunology, Vol. 2, Issue 2, pp. 165-71, 1991 (PubMed).

Tamatani, Kotani, Tanaka, Miyasaka: "Molecular mechanisms underlying lymphocyte recirculation. II. Differential regulation of LFA-1 in the interaction between lymphocytes and high endothelial cells." in: European journal of immunology, Vol. 21, Issue 3, pp. 855-8, 1991 (PubMed).