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  • 产品名称:anti-CD34antibody(CD34Molecule)(PE)

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简单介绍:
anti-CD34antibody(CD34Molecule)(PE)
详情介绍:
Immunogen Canine CD34 fusion protein. Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS-1/FOX-NY myeloma cell line.
Clone 1H6
Isotype IgG1
Specificity This antibody is specific for CD34. Clone 1H6 is reported for use in CD34+ enrichment studies (4,5).
Purification Affinity chromatography on Protein G
Alternative Name CD34 (CD34 Antibody Abstract)
Background CD34 is a cell surface antigen of approximately 110 kDa expressed by endothelial cells and haematopoietic stem cells.Synonyms: Hematopoietic progenitor cell antigen CD34, Hematopoietic progenitor cell marker
Gene ID 415130
NCBI Accession NP_001003341
UniProt Q28270
Research Area Hematopoietic Progenitors, Hematopoietic Stem Cells, Cardiogenesis, Lineage Markers, CD Antigens, Surface Receptors of Immune Cells
Application Notes Flow Cytometry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Reconstitution Restore with 1 mL distilled water
Concentration 0.1 mg/mL
Buffer PBS, pH 7.4 containing 0.09 % Sodium Azide and 1 % Bovine Serum Albumin
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Lyophilized antibody should be stored at 2-8 °C. Reconstituted antibody should be aliquoted and stored at -20 °C or below.
Background publications Horn, Keyser, Peterson, Neff, Thomasson, Thompson, Kiem: "Efficient lentiviral gene transfer to canine repopulating cells using an overnight transduction protocol." in: Blood, Vol. 103, Issue 10, pp. 3710-6, 2004 (PubMed).

Goerner, Horn, Peterson, Kurre, Storb, Rasko, Kiem: "Sustained multilineage gene persistence and expression in dogs transplanted with CD34(+) marrow cells transduced by RD114-pseudotype oncoretrovirus vectors." in: Blood, Vol. 98, Issue 7, pp. 2065-70, 2001 (PubMed).

Bhattacharya, McSweeney, Shi, Bruno, Ishida, Nash, Storb, Sauvage, Hammond, Wu: "Enhanced endothelialization and microvessel formation in polyester grafts seeded with CD34(+) bone marrow cells." in: Blood, Vol. 95, Issue 2, pp. 581-5, 2000 (PubMed).

Goerner, Bruno, McSweeney, Buron, Storb, Kiem: "The use of granulocyte colony-stimulating factor during retroviral transduction on fibronectin fragment CH-296 enhances gene transfer into hematopoietic repopulating cells in dogs." in: Blood, Vol. 94, Issue 7, pp. 2287-92, 1999 (PubMed).

McSweeney, Rouleau, Wallace, Bruno, Andrews, Krizanac-Bengez, Sandmaier, Storb, Wayner, Nash: "Characterization of monoclonal antibodies that recognize canine CD34." in: Blood, Vol. 91, Issue 6, pp. 1977-86, 1998 (PubMed).