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  • 产品名称：anti-S100A9antibody(S100CalciumBindingProteinA9)(C-Term)(Biotin)

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  • 产品厂商：Acris-antibodies
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简单介绍：
anti-S100A9antibody(S100CalciumBindingProteinA9)(C-Term)(Biotin)
详情介绍：
Immunogen Cultured Human Monocytes. Remarks: The antigen is MRP14, the epitope is suspected in the carboxyterminal portion of the peptide.
Clone S32-2
Isotype IgG1
Specificity Monoclonal antibody S32.2 identifies the Ca2+-binding 14 kDa subunit of the inflammatory L-1 protein complex, also called S100A9 or Calgranulin B. It is useful for the characterization of circulating granulocytes or inflammatory infiltrates of the myelo-monocytic lineage which express MRP14 differently depending on the inflammatory status of the disease. S32.2 detects a very distinct epitope on the C-terminal end of MRP14 (different from the S 36.48 epitope). The epitope is stable to 0.1 % glutaraldehyde or formalin fixation, and paraffin embedding. Antigen Distribution Isolated cells: The antigen is found in granulocytes and monocytes. It is absent from all other blood cells. In cultured monocytes, maximum MRP-14 is expressed after 3-4 days. Myeloid leukaemia cells have been found to be positive as well. Tissue Sections: MRP-14 is only found in a distinct subpopulation of inflammatory perivascular infiltrates of the myelo-monocytic lineage. Reports from the literature indicate that macrophages synthesise MRP-14 increasingly during the early stages of inflammation. A high MRP-14 (and low MRP-8) expression by macrophages was also reported in granulomatous diseases such as tuberculosis and sarcoidosis. In non-granulomatous chronic inflammatory diseases such as chronic rheumatoid arthritis MRP-8 and MRP-14 positive cells consist of different subpopulations. During early inflammation endothelial cells are also positive with MRP-8/14 determined by antibody 27E10 (Cat.-No BM4025).Antigen Distribution
Cross-Reactivity (Details) Species reactivity (tested):Human: MRP14, Granulocytes, stimulated Monocytes and Macrophages. Does not react with Bovine and Swine spleen.
Purification Protein A Chromatography
Alternative Name S100A9 / Calgranulin-B / MRP14 (S100A9 Antibody Abstract)
Background S100A9 is a member of the S100 family of proteins. S100A9, together with S100A8 forms a heterodimeric protein complex, Calprotectin, which is a major calcium- and zinc-binding protein in the cytosol of neutrophils, monocytes, and keratinocytes. Complexes of S100A8 and S100A9 are the physiologically relevant forms of these proteins. S100A9 may function in the inhibition of casein kinase and altered expression of this protein is associated with the disease cystic fibrosis. Its expression and potential cytokine-like function in inflammation and in cancer suggest that S100A8/A9 may play a key role in inflammation-associated cancer.Synonyms: CAGB, Calprotectin L1H subunit, Leukocyte L1 complex heavy chain, MRP-14, Migration inhibitory factor-related protein 14, S100 calcium-binding protein A9, S100-A9
Gene ID 9606
UniProt P06702
Pathways Transition Metal Ion Homeostasis, Positive Regulation of Endopeptidase Activity
Application Notes ELISA. Flow Cytometry. Immunohistochemistry (IHC) on: Frozen sections: 0.25 μg/mL (1/800)Paraffin sections: 2 μg/mL (1/100), no pretreatment for antigen retrieval necessary. Suggested positive control: Human tonsil. Has been described to work Dot blots.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Reconstitution Restore in 0.5 mL distilled water to make stock solution.
Concentration 0.2 mg/mL
Buffer PBS, pH 7.2 with 5 mg/mL BSA as a stabilizer and kathon as a preservative
Preservative Kathon CG
Precaution of Use This product contains Kathon CG a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Handling Advice This product is photosensitive and should be protected from light
Storage 4 °C
Storage Comment Store the antibody undiluted undiluted at 2-8 °C. DO NOT FREEZE!
Product cited in: Mortezai, Harder, Schnabel, Moors, Gauly, Schlüter, Wagener, Buck: "Tandem affinity depletion: a combination of affinity fractionation and immunoaffinity depletion allows the detection of low-abundance components in the complex proteomes of body fluids." in: Journal of proteome research, Vol. 9, Issue 12, pp. 6126-34, 2010 (PubMed).

Rosenberger, Thorey, Werner, Boukamp: "A novel regulator of telomerase. S100A8 mediates differentiation-dependent and calcium-induced inhibition of telomerase activity in the human epidermal keratinocyte line HaCaT." in: The Journal of biological chemistry, Vol. 282, Issue 9, pp. 6126-35, 2007 (PubMed).

Background publications Wen, Xu, Mais, Goldman, McDonnell: "The A and B isoforms of the human progesterone receptor operate through distinct signaling pathways within target cells." in: Molecular and cellular biology, Vol. 14, Issue 12, pp. 8356-64, 1994 (PubMed).

Teigelkamp, Bhardwaj, Roth, Meinardus-Hager, Karas, Sorg: "Calcium-dependent complex assembly of the myeloic differentiation proteins MRP-8 and MRP-14." in: The Journal of biological chemistry, Vol. 266, Issue 20, pp. 13462-7, 1991 (PubMed).

Delabie, de Wolf-Peeters, van den Oord, Desmet: "Differential expression of the calcium-binding proteins MRP8 and MRP14 in granulomatous conditions: an immunohistochemical study." in: Clinical and experimental immunology, Vol. 81, Issue 1, pp. 123-6, 1990 (PubMed).

Mues, Brisse, Zwadlo, Themann, Bender, Sorg: "Phenotyping of macrophages with monoclonal antibodies in endomyocardial biopsies as a new approach to diagnosis of myocarditis." in: European heart journal, Vol. 11, Issue 7, pp. 619-27, 1990 (PubMed).

Brandtzaeg, Jones, Flavell, Fagerhol: "Mac 387 antibody and detection of formalin resistant myelomonocytic L1 antigen." in: Journal of clinical pathology, Vol. 41, Issue 9, pp. 963-70, 1989 (PubMed).

Hogg, Allen, Edgeworth: "Monoclonal antibody 5.5 reacts with p8,14, a myeloid molecule associated with some vascular endothelium." in: European journal of immunology, Vol. 19, Issue 6, pp. 1053-61, 1989 (PubMed).

Murao, Collart, Huberman: "A protein containing the cystic fibrosis antigen is an inhibitor of protein kinases." in: The Journal of biological chemistry, Vol. 264, Issue 14, pp. 8356-60, 1989 (PubMed).

Zwadlo, Brüggen, Gerhards, Schlegel, Sorg: "Two calcium-binding proteins associated with specific stages of myeloid cell differentiation are expressed by subsets of macrophages in inflammatory tissues." in: Clinical and experimental immunology, Vol. 72, Issue 3, pp. 510-5, 1988 (PubMed).

Odink, Cerletti, Brüggen, Clerc, Tarcsay, Zwadlo, Gerhards, Schlegel, Sorg: "Two calcium-binding proteins in infiltrate macrophages of rheumatoid arthritis." in: Nature, Vol. 330, Issue 6143, pp. 80-2, 1987 (PubMed).