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  • 产品名称:anti-Macrophage/Monocyteantibody(FITC)

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简单介绍:
anti-Macrophage/Monocyteantibody(FITC)
详情介绍:
Immunogen Mouse lymph node stroma. Remarks: Spleen cells from immunised Wistar rats were fused with cells of the SP/0 myeloma cell line.
Clone MOMA-2
Isotype IgG2b
Specificity The antibody recognizes an intracellular antigen of Mouse Macrophages and Monocytes. It reacts strongly with Macrophages in lymphoid organs such as tingible body Macrophages and Macrophages in T cell dependent areas. Reacts on all Mouse strains tested.
Cross-Reactivity (Details) Species reactivity (tested):Mouse.
Purification Affinity Chromatography on Protein G
Alternative Name Macrophages / Monocytes
Background Monocyte and macrophage are white blood cells that roam the body tissues engulfing foreign organisms. A monocyte is a leukocyte, part of the human body's immune system that protects against blood-borne pathogens and moves quickly (aprox. 8-12 hours) to sites of infection in the tissues. Monocytes are usually identified in stained smears by their large bi-lobed nucleus. Macrophages are cells within the tissues that originate from specific white blood cells called monocytes. Monocytes and macrophages are phagocytes, acting in both nonspecific defense (or innate immunity) as well as specific defense (or cell-mediated immunity) of vertebrate animals. Their role is to phagocytize (engulf and then digest) cellular debris and pathogens either as stationary or mobile cells, and to stimulate lymphocytes and other immune cells to respond to the pathogen.Synonyms: Macrophage marker, Monocyte marker
Application Notes Flow Cytometry: Use 10 μL of neat-1/10 diluted antibody to label 10^6 cells in 100 μL (membrane permeabilisation is recommended).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 0.1 mg/mL
Buffer PBS containing 0.09 % Sodium Azide as preservative and 1 % BSA as stabilizer.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Supplier Images
 image for anti-Macrophage/Monocyte antibody (FITC) (ABIN118286) Staining of mouse peritoneal macrophages cells with RAT ANTI MOUSE MACRO / MONO:FITC ...
Background publications Frossard, Lenglet, Montecucco, Steffens, Galan, Pelli, Spahr, Mach, Hadengue: "Role of CCL-2, CCR-2 and CCR-4 in cerulein-induced acute pancreatitis and pancreatitis-associated lung injury." in: Journal of clinical pathology, Vol. 64, Issue 5, pp. 387-93, 2011 (PubMed).

Yamamoto, Zuo, Ma, Yancey, Hunley, Motojima, Fogo, Linton, Fazio, Ichikawa, Kon: "Oral activated charcoal adsorbent (AST-120) ameliorates extent and instability of atherosclerosis accelerated by kidney disease in apolipoprotein E-deficient mice." in: Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association, Vol. 26, Issue 8, pp. 2491-7, 2011 (PubMed).

de Jager, Bermúdez, Bot, Koenen, Bot, Kavelaars, de Waard, Heijnen, Muriana, Weber, van Berkel, Kuiper, Lee, Abia, Biessen: "Growth differentiation factor 15 deficiency protects against atherosclerosis by attenuating CCR2-mediated macrophage chemotaxis." in: The Journal of experimental medicine, Vol. 208, Issue 2, pp. 217-25, 2011 (PubMed).

Weingärtner, Ulrich, Lütjohann, Ismail, Schirmer, Vanmierlo, Böhm, Laufs: "Differential effects on inhibition of cholesterol absorption by plant stanol and plant sterol esters in apoE-/- mice." in: Cardiovascular research, Vol. 90, Issue 3, pp. 484-92, 2011 (PubMed).

Skoura, Michaud, Im, Thangada, Xiong, Smith, Hla: "Sphingosine-1-phosphate receptor-2 function in myeloid cells regulates vascular inflammation and atherosclerosis." in: Arteriosclerosis, thrombosis, and vascular biology, Vol. 31, Issue 1, pp. 81-5, 2010 (PubMed).

Madrigal-Matute, López-Franco, Blanco-Colio, Muñoz-García, Ramos-Mozo, Ortega, Egido, Martín-Ventura: "Heat shock protein 90 inhibitors attenuate inflammatory responses in atherosclerosis." in: Cardiovascular research, Vol. 86, Issue 2, pp. 330-7, 2010 (PubMed).

Duewell, Kono, Rayner, Sirois, Vladimer, Bauernfeind, Abela, Franchi, Nuñez, Schnurr, Espevik, Lien, Fitzgerald, Rock, Moore, Wright, Hornung, Latz: "NLRP3 inflammasomes are required for atherogenesis and activated by cholesterol crystals." in: Nature, Vol. 464, Issue 7293, pp. 1357-61, 2010 (PubMed).

Bhatia, Yun, Leung, Grimsditch, Benson, Botto, Boyle, Haskard: "Complement C1q reduces early atherosclerosis in low-density lipoprotein receptor-deficient mice." in: The American journal of pathology, Vol. 170, Issue 1, pp. 416-26, 2007 (PubMed).

Bourdillon, Randon, Barek, Zibara, Covacho, Poston, Chignier, McGregor: "Reduced atherosclerotic lesion size in P-selectin deficient apolipoprotein E-knockout mice fed a chow but not a fat diet." in: Journal of biomedicine & biotechnology, Vol. 2006, Issue 2, pp. 49193, 2006 (PubMed).

Nakai, Iwabuchi, Fujii, Ishimori, Dashtsoodol, Watano, Mishima, Iwabuchi, Tanaka, Bezbradica, Nakayama, Taniguchi, Miyake, Yamamura, Kitabatake, Joyce, Van Kaer, Onoé: "Natural killer T cells accelerate atherogenesis in mice." in: Blood, Vol. 104, Issue 7, pp. 2051-9, 2004 (PubMed).

Gal, Tamir, Tannenbaum, Wogan: "Nitric oxide production in SJL mice bearing the RcsX lymphoma: a model for in vivo toxicological evaluation of NO." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 93, Issue 21, pp. 11499-503, 1996 (PubMed).

Kraal, Rep, Janse: "Macrophages in T and B cell compartments and other tissue macrophages recognized by monoclonal antibody MOMA-2. An immunohistochemical study." in: Scandinavian journal of immunology, Vol. 26, Issue 6, pp. 653-61, 1988 (PubMed).