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  • 产品名称:anti-CD8Bantibody(CD8bMolecule)(FITC)

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  • 产品厂商:Acris-antibodies
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简单介绍:
anti-CD8Bantibody(CD8bMolecule)(FITC)
详情介绍:
Immunogen Rat/mouse T cell hybrids expressing CD8. Spleen cells from immunised BALB/c mice were fused with cells of the mouse X63 Ag.653 myeloma cell line.
Clone 341
Isotype IgG1
Specificity This antibody recognises the b chain of the CD8 cell surface antigen. This antibody is reported to block proliferative and cytotoxic responses of CD8 +ve effector cells in vitro.
Purification Affinity chromatography on Protein G
Alternative Name CD8 beta (CD8B Antibody Abstract)
Background CD8b is expressed on thymocytes and peripheral cytotoxic/suppressor cells. CD8b is not expressed by NK cells or by intestinal intraepithelial T cells.Synonyms: CD8B, CD8B1, T-cell surface glycoprotein CD8 beta chain
Gene ID 24931
NCBI Accession NP_113727
UniProt P05541
Research Area CD Antigens, Surface Receptors of Immune Cells
Pathways TCR Signaling
Application Notes Flow cytometry: 1/2 - 1/10, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 0.1 mg/mL
Buffer PBS, pH 7.4 containing 0.09 % Sodium Azide and 1 % Bovine Serum Albumin
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Background publications Torres-Nagel, Kraus, Brown, Tiefenthaler, Mitnacht, Williams, Hünig: "Differential thymus dependence of rat CD8 isoform expression." in: European journal of immunology, Vol. 22, Issue 11, pp. 2841-8, 1992 (PubMed).