产品详情
所在位置: 首页> 产品目录> 一抗>
查看大图

  • 产品名称：anti-MUC1antibody(Mucin1,CellSurfaceAssociated)(FITC)

  • 产品型号：
  • 产品厂商：Acris-antibodies
  • 产品文档：
你添加了1件商品 查看购物车
简单介绍：
anti-MUC1antibody(Mucin1,CellSurfaceAssociated)(FITC)
详情介绍：
Immunogen Urinary MUC-1 mucin. Spleen cells from immunised Balb/c mice were fused with cells of a mouse myeloma cell line.
Clone C595
Isotype IgG3
Specificity This antibody recognises CD227, the breast cancer associated mucin encoded by the Muc-1 gene. In normal tissues expression is restricted to specialised glandular epithelial cells. Clone C595 recognises the peptide epitope ARG-PRO-ALA-PRO within the protein core of the Mucin.
Purification Affinity Chromatography on Protein G
Alternative Name CD227 / Mucin-1 / MUC1 (MUC1 Antibody Abstract)
Background MUC1 is a large cell surface mucin glycoprotein expressed by most glandular and ductal epithelial cells and some hematopoietic cell lineages. It is expressed on most secretory epithelium, including mammary gland and some hematopoietic cells. It is expressed abundantly in lactating mammary glands and overexpressed abundantly in >90 % breast carcinomas and metastases. Transgenic MUC1 has been shown to associate with all four cebB receptors and localize with erbB1 (EGFR) in lactating glands. The MUC1 gene contains seven exons and produces several different alternatively spliced variants. The major expressed form of MUC1 uses all seven exons and is a type 1 transmembrane protein with a large extracellular tandem repeat domain. The tandem repeat domain is highly O glycosylated and alterations in glycosylation have been shown in epithelial cancer cells.Synonyms: Breast carcinoma-associated antigen DF3, CA 15-3, Carcinoma-associated mucin, DF3, EMA, Episialin, H23AG, MUC-1, PEMT, PUM, Peanut-reactive urinary mucin, Polymorphic epithelial mucin, Tumor-associated epithelial membrane antigen, Tumor-associated mucin
Gene ID 4582
NCBI Accession NP_001018016
UniProt P15941
Research Area CD Antigens, Surface Receptors of Immune Cells
Pathways Negative Regulation of intrinsic apoptotic Signaling
Application Notes Flow cytometry: neat - 1/10, use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 0.1 mg/mL
Buffer PBS, pH 7.4 containing 0.09 % Sodium Aziden as preservative and 1 % BSA as stabilizer.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Background publications Denton, Sekowski, Spencer, Hughes, Murray, Denley, Tendler, Price: "Production and characterization of a recombinant anti-MUC1 scFv reactive with human carcinomas." in: British journal of cancer, Vol. 76, Issue 5, pp. 614-21, 1997 (PubMed).

Denton, Davies, Scanlon, Tendler, Price: "Primary sequence determination and molecular modelling of the variable region of an antiMUC1 mucin monoclonal antibody." in: European journal of cancer (Oxford, England : 1990), Vol. 31A, Issue 2, pp. 214-21, 1995 (PubMed).

Price, Sekowski, Tendler: "Purification of anti-epithelial mucin monoclonal antibodies by epitope affinity chromatography." in: Journal of immunological methods, Vol. 139, Issue 1, pp. 83-90, 1991 (PubMed).

Price, Hudecz, OSullivan, Baldwin, Edwards, Tendler: "Immunological and structural features of the protein core of human polymorphic epithelial mucin." in: Molecular immunology, Vol. 27, Issue 8, pp. 795-802, 1990 (PubMed).

Price, Pugh, Hudecz, Griffiths, Jacobs, Symonds, Clarke, Chan, Baldwin: "C595--a monoclonal antibody against the protein core of human urinary epithelial mucin commonly expressed in breast carcinomas." in: British journal of cancer, Vol. 61, Issue 5, pp. 681-6, 1990 (PubMed).