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  • 产品名称：anti-C-MYCantibody(V-MycMyelocytomatosisViralOncogeneHomolog(Avian))(C-Term)(Al

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  • 产品厂商：Acris-antibodies
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简单介绍：
anti-C-MYCantibody(V-MycMyelocytomatosisViralOncogeneHomolog(Avian))(C-Term)(AlkalinePhosphatase(AP))
详情介绍：
Immunogen Synthetic peptide sequence (AEEQKLISEEDLL) corresponding to the C-terminal region of Human c-myc. Spleen cells from immunised Balb/c mice were fused with cells of the myeloma cell line.
Sequence EQKLISEEDL
Clone 9-00E-010
Isotype IgG1
Specificity This antibody detects the 62 kDa c-myc gene product, which is involved in the regulation of the cell cycle and cell growth. This antibody may also be used to detect the commonly used c-Myc 'Tag'.
Cross-Reactivity (Details) Species reactivity (tested):Human.
Purification Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Alternative Name C-Myc (MYC Antibody Abstract)
Background P62c-myc is primarily located to the cell nucleus, but has also been shown to localised to the cytoplasm in several cell lines. Overexpression of c-myc has been reported in a wide variety of human cancers.Synonyms: c-myc tag, myc tag, myc-tag
Gene ID 4609
NCBI Accession NP_002458
UniProt P01106
Research Area Transcription Factors, Cancer, Cell Cycle
Pathways p53 Signaling, Cell Division Cycle, Sensory Perception of Sound, Transition Metal Ion Homeostasis, Mitotic G1-G1/S Phases, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus
Application Notes ELISA: 1/500-1/5000. Western Blot: 1/100-1/500 (Non-Reducing Conditions). Detects a band of approximately 62 kDa in Colo 320HSR cell lysates. Immunohistochemistry on Frozen and Paraffin Sections: This product does not requireprotein digestion pre-treatment of paraffin sections prior to staining. It does not requireantigen retrieval using heat treatment prior to staining of paraffin sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer PBS, 0.09 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store the antibody undiluted at 2-8 °C. DO NOT FREEZE!
Background publications Borodina, Jensen, Søndergaard, Poulsen: "Display of wasp venom allergens on the cell surface of Saccharomyces cerevisiae." in: Microbial cell factories, Vol. 9, pp. 74, 2010 (PubMed).

Head, Griparic, Amiri, Gandre-Babbe, van der Bliek: "Inducible proteolytic inactivation of OPA1 mediated by the OMA1 protease in mammalian cells." in: The Journal of cell biology, Vol. 187, Issue 7, pp. 959-66, 2009 (PubMed).

Krauss, Wessner, Welfle, Welfle, Scholz, Seifert, Zubow, Aÿ, Hahn, Scheerer, Skerra, Höhne: "The structure of the anti-c-myc antibody 9E10 Fab fragment/epitope peptide complex reveals a novel binding mode dominated by the heavy chain hypervariable loops." in: Proteins, Vol. 73, Issue 3, pp. 552-65, 2008 (PubMed).

Groeger, Nobes: "Co-operative Cdc42 and Rho signalling mediates ephrinB-triggered endothelial cell retraction." in: The Biochemical journal, Vol. 404, Issue 1, pp. 23-9, 2007 (PubMed).

Hilpert, Hansen, Wessner, Küttner, Welfle, Seifert, Höhne: "Anti-c-myc antibody 9E10: epitope key positions and variability characterized using peptide spot synthesis on cellulose." in: Protein engineering, Vol. 14, Issue 10, pp. 803-6, 2001 (PubMed).

Spandidos, Pintzas, Kakkanas, Yiagnisis, Mahera, Patra, Agnantis: "Elevated expression of the myc gene in human benign and malignant breast lesions compared to normal tissue." in: Anticancer research, Vol. 7, Issue 6, pp. 1299-304, 1988 (PubMed).

Evan, Lewis, Ramsay, Bishop: "Isolation of monoclonal antibodies specific for human c-myc proto-oncogene product." in: Molecular and cellular biology, Vol. 5, Issue 12, pp. 3610-6, 1986 (PubMed).