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  • 产品名称：anti-CD8antibody(PE)

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  • 产品厂商：Acris-antibodies
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简单介绍：
anti-CD8antibody(PE)
详情介绍：
Clone CT-CD8a
Isotype IgG2a
Specificity This anti-mouse CD8a antigen monoclonal antibody recognizes the mouse CD8α chain. The α chain of CD8 associates with the CD8β chain to form a CD8α/β heterodimer that is expressed by the majority of thymocytes and by the MHC class I restricted subset of mature T cells1. Mouse CD8α can also form a CD8α/α chain homodimer on subsets of CD8 positive cells. For this reason, antibodies specific for CD8a rather than CD8b are recommended for a rigorous delineation of CD8 positive cells.
Background The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell to cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains.Synonyms: CD8 alpha chain, CD8A, MAL, T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2
Gene ID 12525
NCBI Accession NP_001074579
UniProt P01731
Research Area Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells
Application Notes Flow Cytometry Analysis (see Protocols). (Reported to be useful in immunohistochemistry on acetone-fixed frozen sections. )
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Protocol FLOW CYTOMETRY ANALYSIS: Method: 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cellpopulation with Lympholyte®-M cell separation medium. 2. Wash 2 times. 3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 µl of thissuspension to each tube (each tube will then contain 1 x 10e6 cells, representing 1 test). 4. To each tube, add ~1. 0 µg*of this Ab per 1x10e6 cells. 5. Vortex the tubes to ensure thorough mixing of antibody and cells. 6. Incubate the tubes for 30 minutes at 4°C. (It is recommended that the tubes areprotected from light, since most fluorochromes are light sensitive. )7. Wash 2 times at 4°C. 8. Resuspend the cell pellet in 50 µl ice cold media B. 9. Transfer to suitable tubes for flow cytometric analysis containing 15 µl of propidiumiodide at 0. 5 mg/ml in PBS. This stains dead cells by intercalating in DNA. Media: A. Phosphate buffered saline (pH 7. 2) + 5% normal serum of host species + sodium azide(100 µl of 2M sodium azide in 100 mls). B. Phosphate buffered saline (pH 7. 2) + 0. 5% Bovine serum albumin + sodium azide (100µl of 2M sodium azide in 100 mls). Results: Mouse Strain: BALB/cCell Concentration: 1x10e6 cells per testAntibody Concentration Used: 1. 0 µg/10e6 cells
Restrictions For Research Use only
Concentration 0.1 mg/mL
Buffer PBS, 0.1 % sodium azide (NaN3) and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/mL
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store the antibody undiluted at 2-8 °C. DO NOT FREEZE! This Antibody is photosensitive and should be protectet from light.
Supplier Images
Flow Cytometry (FACS) image for anti-CD8 antibody (PE) (ABIN114176) Representative Histogram - Isotypic Control: PE Rat IgG2a Percentage of cells stained...
Background publications Weber, Shi, Heise, Warner, Mahvi: "Interleukin-12 gene transfer results in CD8-dependent regression of murine CT26 liver tumors." in: Annals of surgical oncology, Vol. 6, Issue 2, pp. 186-94, 1999 (PubMed).

Tomonari, Spencer: "Epitope-specific binding of CD8 regulates activation of T cells and induction of cytotoxicity." in: International immunology, Vol. 2, Issue 12, pp. 1189-94, 1991 (PubMed).