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  • 产品名称：anti-Podoplaninantibody(PDPN)(Biotin)

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  • 产品厂商：Acris-antibodies
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简单介绍：
anti-Podoplaninantibody(PDPN)(Biotin)
详情介绍：
Immunogen Podoplanin-expressing MDCK cells.
Clone 18H5
Isotype IgG1
Specificity This antibody reacts with Podoplanin (gp36). The unconjugated monoclonal antibody will detect native Human Podoplanin in Western Blot and on the surface of Human LEC.
Cross-Reactivity (Details) Species reactivity (tested):Human.
Purification Affinity Chromatography on Protein G
Alternative Name Podoplanin (PDPN Antibody Abstract)
Background Podoplanin, also known as glycoprotein 36 (gp36), PA2.26 antigen, T1alpha (T1A), and aggrus, is a 36 kDa type I transmembrane sialoglycoprotein and member of the Podoplanin family. Podoplanin has three potential splice variants, the longest of which is represented by a 238 amino acid (aa) precursor (NP_006465). It contains an undefined signal sequence, a 22 aa transmembrane segment (aa 207228) and a short cytoplasmic tail (aa 229-238). The ECD contains abundant Ser/Thr residues that could serve as potential Olinked glycosolation sites. The cytoplasmic tail contains putative sites for protein kinase C phosphorylation. There are two potential alternate start sites at Met 77 (Swiss Prot #: Q86YL7) and Met 119 (EAW51692) that generate short forms. The 162 aa short form Podoplanin precursor shares 47 % aa identity with mouse Podoplanin. Podoplanin is expressed on glomerular epithelial cells (podocytes), type I lung alveolar cells, lymphatic endothelial cells, and numerous tumors, including colorectal tumors, squamous cell carcinomas, testicular seminoma, and brain tumors. One study shows high expression of Podoplanin mRNA in placenta, lung, skeletal muscle, and heart, and weaker levels in brain, kidney, and liver. Podoplanin is the ligand for Ctype lectin-like receptor 2 (CLEC2). Their association is dependent on sialic acid on Oglycans of Podoplanin. Through its association with CLEC2, Podoplanin-induces platelet aggregation and tumor metastasis. Podoplanin is also necessary for lymphatic vessel formation, normal lung cell proliferation and alveolus formation at birth.Synonyms: Aggrus, GP36, Glycoprotein 36, PA2.26 antigen, PDPN, PSEC0003, PSEC0025, T1-alpha
Gene ID 10630
NCBI Accession NP_001006625
UniProt Q86YL7
Pathways Dicarboxylic Acid Transport
Application Notes Flow cytometry: use at 2-5 μg/mL together with the appropriate secondary reagents.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Reconstitution Restore in sterile water to a concentration of 0.1-1.0 mg/mL. Centrifuge vial prior to opening.
Buffer PBS, 0.02 % Sodium Azide, BSA (50x)
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody at -20 °C.
Product cited in: Conrad, Niess, Huss, Huber, von Luettichau, Nelson, Ott, Jauch, Bruns: "Multipotent mesenchymal stem cells acquire a lymphendothelial phenotype and enhance lymphatic regeneration in vivo." in: Circulation, Vol. 119, Issue 2, pp. 281-9, 2009 (PubMed).

Turner, Langley, Johnston, Gentil, Ford, Wu, Graham, Sharpley, Slatko, Pearlman, Taylor: "Wolbachia lipoprotein stimulates innate and adaptive immunity through Toll-like receptors 2 and 6 to induce disease manifestations of filariasis." in: The Journal of biological chemistry, Vol. 284, Issue 33, pp. 22364-78, 2009 (PubMed).

Kilic, Oliveira-Ferrer, Neshat-Vahid, Irmak, Obst-Pernberg, Wurmbach, Loges, Kilic, Weil, Lauke, Tilki, Singer, Ergün: "Lymphatic reprogramming of microvascular endothelial cells by CEA-related cell adhesion molecule-1 via interaction with VEGFR-3 and Prox1." in: Blood, Vol. 110, Issue 13, pp. 4223-33, 2007 (PubMed).

Background publications Schacht, Ramirez, Hong, Hirakawa, Feng, Harvey, Williams, Dvorak, Dvorak, Oliver, Detmar: "T1alpha/podoplanin deficiency disrupts normal lymphatic vasculature formation and causes lymphedema." in: The EMBO journal, Vol. 22, Issue 14, pp. 3546-56, 2003 (PubMed).

Zimmer, Oeffner, Von Messling, Tschernig, Gröness, Klenk, Herrler: "Cloning and characterization of gp36, a human mucin-type glycoprotein preferentially expressed in vascular endothelium." in: The Biochemical journal, Vol. 341 ( Pt 2), pp. 277-84, 1999 (PubMed).

Breiteneder-Geleff, Matsui, Soleiman, Meraner, Poczewski, Kalt, Schaffner, Kerjaschki: "Podoplanin, novel 43-kd membrane protein of glomerular epithelial cells, is down-regulated in puromycin nephrosis." in: The American journal of pathology, Vol. 151, Issue 4, pp. 1141-52, 1997 (PubMed).