产品详情
所在位置: 首页> 产品目录> 一抗>
查看大图

  • 产品名称：anti-M13Bacteriophagegp3antibody(Biotin)

  • 产品型号：
  • 产品厂商：Acris-antibodies
  • 产品文档：
你添加了1件商品 查看购物车
简单介绍：
anti-M13Bacteriophagegp3antibody(Biotin)
详情介绍：
Immunogen Hybridoma produced by the fusion of splenocytes from mice immunized with isolated M13phage coat proteins and mouse myeloma cells.
Clone E1
Isotype IgG2a
Specificity This antibody recognizes M13 bacteriophage (gp3).
Characteristics Synonyms: Attachment protein G3P, Gene 3 protein, Minor coat protein A
Purification Protein A/G Chromatography
Alternative Name M13 Bacteriophage G3P Minor Coat
Background The display of repertoires of antibody fragments on the surface of filamentous phage offers a new way to produce immunoreagents with defined specificities. Phage derived antibody fragments offer a number of advantages over mouse monoclonal antibodies, such as better clearance from the blood, the possibility to select from human combinatorial libraries and the relative ease by which such fragments can be manipulated. The phage display technique thus facilitates the selection of antibody fragments of therapeutic value or research interest. Antibodies to M13 filamentous phage coat proteins are instrumental in the selection and detection of phages expressing specific antibody fragments or peptide sequences at their surface.Synonyms: Attachment protein G3P, Gene 3 protein, Minor coat protein A
Gene ID 927334
UniProt P69168
Application Notes Immunohistochemistry. Western blot: 1 - 5 μg/mL. Flow cytometry: 1 g/10^6 cells. ELISA.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer Phosphate buffered saline with 0.08 % Sodium Azide as preservative
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment The antibody can be shipped at ambient temperature. Store (in aliquots) at -20 °C only. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Expiry Date 12 months
Supplier Images
image for anti-M13 Bacteriophage gp3 antibody (Biotin) (ABIN264521) anti-M13 Bacteriophage gp3 antibody (Biotin)
Background publications Beckmann, Haase, Timmis, Tesar: "Multifunctional g3p-peptide tag for current phage display systems." in: Journal of immunological methods, Vol. 212, Issue 2, pp. 131-8, 1998 (PubMed).

Ebright, Dong, Messing: "Corrected nucleotide sequence of M13mp18 gene III." in: Gene, Vol. 114, Issue 1, pp. 81-3, 1992 (PubMed).

Yanisch-Perron, Vieira, Messing: "Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors." in: Gene, Vol. 33, Issue 1, pp. 103-19, 1985 (PubMed).

Zoller, Smith: "Oligonucleotide-directed mutagenesis of DNA fragments cloned into M13 vectors." in: Methods in enzymology, Vol. 100, pp. 468-500, 1983 (PubMed).

Hong: "A method for sequencing single-stranded cloned DNA in both directions." in: Bioscience reports, Vol. 1, Issue 3, pp. 243-52, 1982 (PubMed).

Hu, Messing: "The making of strand-specific M13 probes." in: Gene, Vol. 17, Issue 3, pp. 271-7, 1982 (PubMed).

Cleary, Ray: "Deletion analysis of the cloned replication origin region from bacteriophage M13." in: Journal of virology, Vol. 40, Issue 1, pp. 197-203, 1981 (PubMed).

Hines, Ray: "Construction and characterization of new coliphage M13 cloning vectors." in: Gene, Vol. 11, Issue 3-4, pp. 207-18, 1981 (PubMed).

Heidecker, Messing, Gronenborn: "A versatile primer for DNA sequencing in the M13mp2 cloning system." in: Gene, Vol. 10, Issue 1, pp. 69-73, 1980 (PubMed).

van Wezenbeek, Schoenmakers: "Nucleotide sequence of the genes III, VI and I of bacteriophage M13." in: Nucleic acids research, Vol. 6, Issue 8, pp. 2799-818, 1979 (PubMed).

Sanger, Nicklen, Coulson: "DNA sequencing with chain-terminating inhibitors." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 74, Issue 12, pp. 5463-7, 1978 (PubMed).