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  • 产品名称：HIV-1Tat ELISA Kit

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  • 产品厂商：DIATHEVA
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简单介绍：
HIV-1Tat ELISA Kit
详情介绍：
Purpose Enzyme linked immunosorbent assay (ELISA) for the detection of anti-tat IgG in human serum and plasma, as an aid in monitoring the progression of disease in HIV-1 infected patients
Sample Type Serum, Plasma
Analytical Method Quantitative
Detection Method Colorimetric
Components Microtiter strips (12 x 8-well strips) coated with tat protein and blocked with BSA Lysis Buffer: 10ml ready to use, with sodium azide Buffer A (Samples diluent): 50ml ready to use, with preservative Buffer A1 (Positive Control diluent): 1.5ml ready to use, with preservative Buffer B (10X Wash Buffer concentrate): 100ml 10X with preservative, to dilute to 1000 ml final volume with distilled water Buffer S (Secondary Antibody diluent): 20ml ready to use, with preservative Conjugated antibody: goat anti-human IgG-HRP conjugated, 10μl Buffer C: 30ml ABTS Tablets: 2 tablets Buffer C1: 30μl Positive Control: 30μl Negative Control: 10μl
Material not included Microplate reader capable of measuring absorbance at 415 nm Thermomixer, shaking water bath or rocking platform at 37 °C Precision pipettes and pipette tips Glass or plastic pipettes Deionized or distilled water Multi-channel pipette, semi-automated or automated microplate washer 1000 mL graduated cylinder for preparation of 1X Wash Buffer Vortex mixer Glass tubes
Alternative Name HIV1 Tat
Background The HIV-1 regulatory tat proteins are considered attractive targets for the development of a multicomponent vaccine against HIV-1 infection. The protein is well conserved among different isolates and thus may be less susceptible to mutation leading to the production of escape virus variants. Tat is produced early after infection and it is essential for virus replication and infectivity. Tat protein is also immunogenic and antibodies against tat have been found to correlate with delayed disease progression and may exert protective effects by inhibiting both HIV replication and the effects of extracellular tat.
Application Notes Optimal working dilution should be determined by the investigator.
Comment

Specifically, it could be useful for the screening and control of eligible patients in vaccination programs of HIV-1 infections with tat.
Plate Pre-coated
Restrictions For Research Use only
Precaution of Use The buffer C1 provided with this kit is H2O2 solution 30% m/m (110 volumes) that causes burns
Storage 4 °C