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1. A pH 6 Citrate buffer or pH 9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.\. ELISA: 50 ng/well - 200 ng/well (Capture), 0.05-0.2 μg/mL (Detection),Immunocytochemistry: 0.5-2 μg/mL,Immunohistochemistry (1): 0.5-2 μg/mL