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Characteristics
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Reaction of the IRDye 650 Azide group with the alkyne group is typically accomplished by copper-catalyzed Click Chemistry, whereas the IRDye 650 Azide group spontaneously reacts with the DBCO group over a wide range of conditions. Within physiologically relevant temperature and pH ranges, IRDye 650 Azide is inert toward naturally occurring nucleophiles such as amines and hydroxyls. However, the azide group is unstable toward sulfhydryls (-SH, thiols) and organophosphines such as tris(2-carboxylethyl)phosphine (TCEP).
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