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  • 产品名称：PPT1 ELISA Kit(Palmitoyl-ProteinThioesterase1)

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  • 产品厂商：国内供应3
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简单介绍：
PPT1 ELISA Kit(Palmitoyl-ProteinThioesterase1)
详情介绍：
Detection Method Colorimetric
Characteristics The ELISA is based on the competitive binding enzyme immunoassaytechnique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to PPT-1. During the reaction, PPT-1 in the sample or standard competes with a fixed amount of biotin-labeled PPT-1 for sites on a pre-coated Monoclonal antibody specific to PPT-1. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of PPT-1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Components Reagent (Quantity): Assay plate (1), Standard (2), Sample Diluent (1x20ml), Assay Diluent A (1x10ml), Assay Diluent B (1x10ml), Detection Reagent A (1x120µl), Detection Reagent B (1x120µl), Wash Buffer(25 x concentrate) (1x30ml), Substrate (1x10ml), Stop Solution (1x10ml)
Alternative Name Palmitoyl-protein thioesterase 1 (PPT1 ELISA Kit Abstract)
Background Synonyms: Homo sapiens,Human,Palmitoyl-protein hydrolase 1,Palmitoyl-protein thioesterase 1,PPT,PPT1,PPT-1
Comment

Gene Name: PPT1
Sample Volume 100 μL
Plate Pre-coated
Restrictions For Research Use only
Storage 4 °C/-20 °C
Storage Comment The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 °C upon being received. The other reagents can be stored at 4 °C.