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  • 产品名称：Activity-DependentNeuroprotectorHomeobox(ADNP) ELISA Kit

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  • 产品厂商：国内供应3
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简单介绍：
Activity-DependentNeuroprotectorHomeobox(ADNP) ELISA Kit
详情介绍：
Purpose This immunoassay kit allows for the in vitro quantitative determination of mouseActivity-dependent neuroprotector homeobox proteinconcentrationsin serum, plasma, tissue homogenates,cell culture supernates, and other biological fluids.
Analytical Method Quantitative
Detection Method Colorimetric
Specificity This assay recognizes recombinant and natural MouseActivity-dependent neuroprotector homeobox protein. No significant cross-reactivity or interference was observed.
Components Reagent (Quantity): Assay plate (1), Standard (2), Sample Diluent (1x20ml), Assay Diluent A (1x10ml), Assay Diluent B (1x10ml), Detection Reagent A (1x60µl), Detection Reagent B (1x120µl), Wash Buffer(25 x concentrate) (1x30ml), Substrate (1x10ml), Stop Solution (1x10ml)
Alternative Name Activity-dependent neuroprotector homeobox protein (ADNP ELISA Kit Abstract)
Background Synonyms: Activity-dependent neuroprotective protein,Activity-dependent neuroprotector homeobox protein,Adnp,Mouse,Mus musculus
Pathways Regulation of Cell Size, Regulation of Carbohydrate Metabolic Process
Comment

Gene Name: Adnp
Sample Volume 100 μL
Plate Pre-coated
Protocol he ELISA is based on the competitive binding enzyme immunoassaytechnique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Activity-dependent neuroprotector homeobox protein, during the reaction,Activity-dependent neuroprotector homeobox proteinin the sample or standard competes with a fixed amount of biotin-labeled Activity-dependent neuroprotector homeobox proteinfor sites on a pre-coated Monoclonal antibody specific to Activity-dependent neuroprotector homeobox protein. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Thena TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentrationof Activity-dependent neuroprotector homeobox proteinin the samples is then determined by comparing the O.D. of the samples to the standard curve.
Restrictions For Research Use only
Storage 4 °C/-20 °C
Storage Comment The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 °C upon being received. The other reagents can be stored at 4 °C.