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  • 产品名称：VitaminB12ImmunoaffinityColumn

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  • 产品厂商：EagleBio
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简单介绍：
VitaminB12ImmunoaffinityColumn
详情介绍：
Purpose Many methods of Vitamin B12 determination are based on HPLC-UV (High Performance Liquid Chromatography-ultraviolet) detection show low selectivity if problematic matrices are applied. This method of content determination of Vitamin B12 combines the high selectivity of immunoaffinity columns with its potential to concentrate, elute, and purify with the HPLC column.
Characteristics Many methods of Vitamin B12 determination are based on HPLC-UV (High Performance Liquid Chromatography-ultraviolet) detection show low selectivity if problematic matrices are applied. This method of content determination of Vitamin B12 combines the high selectivity of immunoaffinity columns with its potential to concentrate, elute, and purify with the HPLC column.
Protocol The measuring range is linear of 25 ng to 1250 ng Vitamin B12 pro injection (R2=0.9999). The limit of detection is 3 ng of vitamin B12 per injection (three times of signal/noise ratio). If the given dilution steps are followed, the vitamin B12 contents of 0.1 to 5 μg/g lie within the linear working range of the method. If the contents of the samples used are higher than the cited range, extracts should be diluted in a suitable manner. The lower limit of detection is 10 ng/g of vitamin B12 in the sample.
Restrictions For Research Use only
Storage 4 °C