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  • 产品名称：Dnmt1Chromobody®plasmid(GFP)

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  • 产品厂商：ChromoTek
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简单介绍：
Dnmt1Chromobody®plasmid(GFP)
详情介绍：
Purpose With the Dnmt1-Chromobody®plasmid you can visualize endogenous Dnmt1 in real time during cell cycle progression in your cell line of interest.
Brand Chromobody®
Specificity All Chromobody® binding domains are carefully selected to not interfere with endogenous protein function and offer the unique possibility to trace native proteins in living cells.
Characteristics Chromobodies® are a novel species of extremely small intracellular functional antibodies.

Chromobodies® are based on the antigen binding domain (VHH) derived from heavy chain antibodies of camelids genetically fused to a fluorescent protein. As opposed to conventional antibodies, these innovative fluorescent nanoprobes are suitable for real time analyses and can visualize endogenous cellular structures and processes in live cells.
Thus, Chromobodies® are the perfect reagents for cellular research including High-Content Analysis (HCA).

The Chromobody® plasmid allows you to create your own cell lines or transiently express the Chromobody®.
Alternative Name Dnmt-1
Background Antibodies - extremely powerful tools in biomedical research - are large complex molecules (150 kDa) consisting of two heavy and two light chains. Due to their complex structure the use of antibodies is often limited.

Camelidae (camels, dromedaries, llamas and alpacas) produce functional antibodies devoid of light chains, so called heavy chain antibodies (hcAbs). hcAbs recognize and bind their antigens via a single variable domain (VHH). These VHH domains are the smallest intact antigen binding fragments (molecular weight: 13 kDa), characterized by excellent stability, high specificity and affinities in the low nanomolar range.

DNA methylation is a crucial part of normal organismal development and cellular differentiation in higher organisms. DNA methylation stably alters the gene expression pattern in cells such that cells can
Application Notes Chromobodies® are a novel species of intracellular functional antibodies. Chromobodies® are made by genetically fusing the antigen binding domain (VHH) derived from heavy chain antibodies of camelids to fluorescent proteins, e.g. TagGFP2 or TagRFP.

With ChromoTek's unique Chromobody®-Technology the discrepancy and limitations of antibodies and fluorescent protein tagged reporters can now be overcome. You are now able to study the endogenous proteins of your target of interest in living cells - instead of artificial fluorescent fusion proteins which may behave differently from the real protein or may even be toxic to the cells. In combination with High-Throughput Microscopy and automated image analysis Chromobodies® set up a new class of intracellular biomarkers for High-Content Analysis (HCA). Our proof-of-principle studies include:
  • cell cycle monitoring
  • real time apoptosis studies
  • detection of posttranslational modification


These reagents thus significantly expand both the quality and the quantity of information that can be gathered in HCA.
Comment

The new Dnmt1-Chromobody® comprises the antigen binding domain (VHH) derived from Alpaca heavy chain antibodies genetically fused to TagGFP2.

Features of the Chromodody:
Trace dynamic changes during the cell cycle in real time
Monitor the distribution of an endogenous cell cycle marker protein - unlike with fluorescent fusion proteins there are no overexpression artifacts or cytotoxic effects
Protocol New way to trace Dnmt1 in live cells non-invasively!

With the Dnmt1-Chromoboy® plasmid you obtain the sequence of the Alpaca antibody to Dnmt1 fused to TagRFP or TagGFP. Upon transformation, your cells express the Dnmt1-Chromobody® and Dnmt1 is visualized by binding of the fluorescent Chromobody®. The transient binding does not influence normal cell/Dnmt1 function and thus offers you the unique possibility to observe endogenous Dnmt1 localization in your cells.
Restrictions For Research Use only
Storage -80 °C
Storage Comment Store at -80°C. Short term storage at -20°C.
Supplier Images
Immunofluorescence (IF) image for Dnmt1 Chromobody® plasmid (GFP) (ABIN1478365) Still images of Dnmt1-Chromobody transfected HeLa cells throughout the cell cycle. Ag...