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  • 产品名称：anti-ProtonATPase(AA826-838)antibody

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  • 产品厂商：SYSY
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简单介绍：
anti-ProtonATPase(AA826-838)antibody
详情介绍：
Immunogen Synthetic peptide FSFEHIREGKFDE (826-838 in rat) coupled to key-hole limpet hemocyanin via an added N-terminal cysteine residue.
Specificity Specific for the alpha1 116kDa subunit.
Purification Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization.
Application Notes WB: 1 : 1000 (AP staining)
IP: not tested yet
ICC: 1 : 100
IHC: 1 : 100
Comment

WB: The proton pump aggregates after boiling, making it necessary to run SDS-PAGE only with non-boiled samples. ICC: Methanol fixation is recommended.
Restrictions For Research Use only
Format Lyophilized
Reconstitution For reconstitution add 50 μL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.
Buffer PBS
Handling Advice Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4?°C for prolonged periods (i.e. several weeks), is not recommended.
Storage -20 °C
Storage Comment Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!
Supplier Images
Western Blotting (WB) image for anti-Proton ATPase (AA 826-838) antibody (ABIN1742217) dilution: 1 : 1000, sample: synaptic vesicle fraction of rat brain (LP2)
Immunocytochemistry (ICC) image for anti-Proton ATPase (AA 826-838) antibody (ABIN1742217) Indirect immunostaining of methanol fixed rat hippocampus neurons (dilution 1 : 100; ...