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  • 产品名称:anti-SynaptotagminII(SYT2)(AA1-11),(LumenalRegion)antibody

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  • 产品厂商:SYSY
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简单介绍:
anti-SynaptotagminII(SYT2)(AA1-11),(LumenalRegion)antibody
详情介绍:
Immunogen Synthetic peptide (aa 1-11 in mouse synaptotagmin 2) coupled to key-hole limpet hemocyanin via an added C-terminal cysteine.
Specificity Specific for synaptotagmin 2.
Purification Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization.
Alternative Name Synaptotagmin 2 (SYT2 Antibody Abstract)
Pathways Synaptic Vesicle Exocytosis
Application Notes WB: 1 : 1000 (AP staining)
ICC: 1 : 100
IHC: not tested yet
Comment

These antibodies can be used for labeling of recycling synaptic vesicles by adding to living neurons or as a marker for exocytosis in isolated nerve terminals.

Restrictions For Research Use only
Format Lyophilized
Reconstitution For reconstitution add 50 μL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.
Buffer PBS
Handling Advice Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4?°C for prolonged periods (i.e. several weeks), is not recommended.
Storage -20 °C
Storage Comment Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!
Supplier Images
Western Blotting (WB) image for anti-Synaptotagmin II (SYT2) (AA 1-11), (Lumenal Region) antibody (ABIN1742203) dilution: 1 : 1000, sample: synaptic vesicle fraction of rat brain (LP2)
Immunocytochemistry (ICC) image for anti-Synaptotagmin II (SYT2) (AA 1-11), (Lumenal Region) antibody (ABIN1742203) Labeling of recycling vesicles in cultured neurons from rat cerebellum (dilution 1 : ...