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  • 产品名称:anti-Synaptojanin1(SYNJ1)(AA1140-1155),(Isoform2)antibody

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  • 产品厂商:SYSY
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简单介绍:
anti-Synaptojanin1(SYNJ1)(AA1140-1155),(Isoform2)antibody
详情介绍:
Immunogen Synthetic peptide CGVGAPPSPGVTRREME (aa 1140-1155 in isoform 2) coupled to key-hole limpet hemocyanin via an added N-terminal cysteine residue.
Specificity Recognizes isoforms 1, 2, 3 of synaptojanin 1.
Purification Affinity purified with the immunogen.
Alternative Name Synaptojanin 1 (SYNJ1 Antibody Abstract)
Pathways Inositol Metabolic Process, Synaptic Vesicle Exocytosis
Application Notes WB: 1 : 200 up to 1 : 2000 (AP staining)
IP: not tested yet
ICC: not tested yet
IHC: 1 : 1000 up to 1 : 5000
Comment

Running SDS-PAGE with non-boiled samples gives stronger signals.

Restrictions For Research Use only
Format Lyophilized
Reconstitution For reconstitution add 50 μL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.
Buffer PBS
Handling Advice Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4?°C for prolonged periods (i.e. several weeks), is not recommended.
Storage -20 °C
Storage Comment Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!
Supplier Images
Immunohistochemistry (IHC) image for anti-Synaptojanin 1 (SYNJ1) (AA 1140-1155), (Isoform 2) antibody (ABIN1742317) Indirect immunostaining of a PFA fixed mouse retina section (dilution 1 : 5000).
Western Blotting (WB) image for anti-Synaptojanin 1 (SYNJ1) (AA 1140-1155), (Isoform 2) antibody (ABIN1742317) dilution: 1 : 200, sample: crude synaptosomal fraction of rat brain (P2)