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  • 产品名称：anti-Profilin(PFN)(AA42-47)antibody

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简单介绍：
anti-Profilin(PFN)(AA42-47)antibody
详情介绍：
Immunogen Recombinant birch profilin. Epitope PQFKPQ (aa 42-47)
Clone 4A6
Isotype IgG2a
Specificity Specific for birch profilin.
Purification purified IgG
Alternative Name Profilin
Application Notes WB: 1 : 500 up to 1 : 10000
IHC: not tested yet
Comment

This antibody has been used successfully with eucaryotic and procaryotic expression vectors using the epitope as a tag (Rudiger et al. 1997).
Restrictions For Research Use only
Format Lyophilized
Reconstitution For reconstitution add 100 μL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.
Buffer PBS
Handling Advice Do not store diluted antibody solutions unless you add detergent or carrier proteins such as goat serum, BSA or others. IgG sticks to glass and plastic. Any IgG solution below 0.1?mg/mL protein will quickly adsorb and denature and thus loose activity! Repetitive freeze-thawing of dilute purified IgG is almost certain to lead to substantial losses.
Storage -20 °C
Storage Comment Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.
Product cited in: Wiedemann, Giehl, Almo, Fedorov, Girvin, Steinberger, Rüdiger, Ortner, Sippl, Dolecek, Kraft, Jockusch, Valenta: "Molecular and structural analysis of a continuous birch profilin epitope defined by a monoclonal antibody." in: The Journal of biological chemistry, Vol. 271, Issue 47, pp. 29915-21, 1997 (PubMed).